HA/Hemagglutinin 蛋白, H5N1 (AAT76166, sf9, His)

HA/Hemagglutinin Protein is a viral membrane protein responsible for the initial steps of the entry of influenza virus into the host cell.It mediates binding of the virus particle to the host-cell membrane and catalyzes fusion of the viral membrane with that of the host.HA is pivotal in determining virus host range and virulence.HA/Hemagglutinin Protein, H5N1 (AAT76166, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

血凝素蛋白 (HA) 是一种 I 类病毒融合蛋白，与细胞表面上含唾液酸的受体结合，促进病毒颗粒附着到细胞上。这种附着通过网格蛋白依赖性内吞作用诱导约三分之二的病毒颗粒内化，并通过网格蛋白和小窝蛋白独立途径诱导约三分之一的病毒颗粒内化。HA 在确定病毒的宿主范围限制和毒力方面也起着至关重要的作用。通过介导内吞病毒颗粒膜与内体膜的融合，HA 使病毒能够渗透到细胞质中。在内体的低pH环境下，HA2发生不可逆的构象变化，释放融合疏水肽并形成有能力的融合孔。HA 作为由二硫键连接的 HA1-HA2 亚基组成的同源三聚体存在，并与人 CACNA1C 相互作用^[1][2][3]。

Measured by its ability to agglutinate guinea pig red blood cells.

Virus

Sf9 insect cells

C-His

AAT76166 (D17-Q531, with cleavage site mutated RERRRKKR to TETR)

/

Approximately 59.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.