1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA1/Hemagglutinin 蛋白, H5N1 (H8PF45, HEK293, His)

HA1/Hemagglutinin 蛋白, H5N1 (H8PF45, HEK293, His)

目录号: HY-P73980
产品使用指南

HA/血凝素蛋白在病毒生命周期中至关重要,它与唾液酸受体结合并促进病毒附着于宿主细胞。这种相互作用通过网格蛋白依赖性或不依赖性途径促进病毒粒子内化。HA1/Hemagglutinin 蛋白, H5N1 (H8PF45, HEK293, His) 是重组的 HA/血凝素蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

The HA/hemagglutinin protein is critical in the viral life cycle, binding to sialic acid receptors and facilitating viral attachment to host cells. This interaction promotes virion internalization through clathrin-dependent or -independent pathways. HA/Hemagglutinin Protein, H5N1 (H8PF45, HA1, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

研究背景

HA 蛋白,也称为血凝素蛋白,在病毒生命周期中起着至关重要的作用。它与含有唾液酸的细胞表面受体结合,促进病毒颗粒附着到宿主细胞上。这种附着导致病毒粒子通过网格蛋白依赖性内吞作用途径或网格蛋白和小窝蛋白独立途径内化。HA蛋白是I类病毒融合蛋白,负责介导内吞病毒颗粒膜与内体膜的融合。这种融合事件允许病毒进入细胞的细胞质。内体中的低pH环境会触发蛋白质HA2亚基的不可逆构象变化,导致融合疏水肽的释放。HA 蛋白的多个三聚体对于形成有效的融合孔是必需的。HA蛋白在受体结合、病毒内化、融合和膜穿透方面的功能对于确定病毒的宿主范围限制和毒力至关重要。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

H8PF45 (D17-E340)

基因 ID

/

蛋白结构
N-term
HA1/Hemagglutinin (D17-E340)
Accession # H8PF45
His
C-term
同用名
HA; Hemagglutinin; HA/Hemagglutinin Protein, H5N1 (A/Thailand/1(KAN-1)/2004, HA1, HEK293, His)
分子量

Approximately 38.1 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA1/Hemagglutinin Protein, H5N1 (H8PF45, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA1/Hemagglutinin Protein, H5N1 (H8PF45, HEK293, His)
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HY-P73980
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