1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA1/Hemagglutinin 蛋白, H5N1 (Q692M2, HEK293, His)

HA1/Hemagglutinin 蛋白, H5N1 (Q692M2, HEK293, His)

目录号: HY-P73979
产品使用指南

HA/血凝素蛋白与唾液酸受体结合,启动病毒附着于细胞。这种结合通过网格蛋白依赖性或非依赖性途径促进病毒粒子内化。HA/Hemagglutinin 蛋白, H5N1 (Q692M2, HEK293, His) 是重组的 HA/血凝素蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

The HA/hemagglutinin protein binds to sialic acid receptors, initiating viral attachment to cells. This binding promotes virion internalization via clathrin-dependent or -independent pathways. HA/Hemagglutinin Protein, H5N1 (Q692M2, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

研究背景

HA/血凝素蛋白与细胞表面上含唾液酸的受体结合,促进病毒颗粒附着到细胞上。这种附着通过网格蛋白依赖性内吞作用或网格蛋白和小窝蛋白独立途径触发病毒粒子内化。它对于确定病毒的宿主范围限制和毒力起着至关重要的作用。作为I类病毒融合蛋白,它负责介导内吞病毒颗粒的膜与内体膜的融合,使病毒渗透到细胞的细胞质中。内体中的低pH环境导致HA2发生不可逆的构象变化,导致融合疏水肽的释放。需要多个三聚体才能形成有能力的融合孔。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

Q692M2 (D17-R342)

基因 ID

/

蛋白结构
N-term
HA1/Hemagglutinin (D17-R342)
Accession # Q692M2
His
C-term
同用名
HA; Hemagglutinin; HA/Hemagglutinin Protein, H5N1 (A/chicken/Jilin/9/2004, HEK293, His)
分子量

Approximately 38.2 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA1/Hemagglutinin Protein, H5N1 (Q692M2, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA1/Hemagglutinin Protein, H5N1 (Q692M2, HEK293, His)
目录号:
HY-P73979
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