HA/Hemagglutinin 蛋白, Influenza B (A4D5Q0, sf9, His)

The Hemagglutinin (HA) protein crucially attaches virus particles to host cells by binding to sialic acid-containing receptors, inducing virion internalization through clathrin-dependent endocytosis. HA also facilitates an alternative clathrin- and caveolin-independent pathway for a portion of the virus particles. As a Class I viral fusion protein, HA mediates fusion between the virus particle's membrane and the endosomal membrane, vital for host range restriction and virulence determination, involving a low pH-triggered conformational change in HA2 and the release of the fusion hydrophobic peptide. HA/Hemagglutinin Protein, Influenza B (A4D5Q0, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of HA/Hemagglutinin Protein, Influenza B (A4D5Q0, sf9, His) is 546 a.a., with molecular weight of ~59 kDa.

血凝素 (HA) 蛋白通过与细胞表面含唾液酸的受体结合，在病毒颗粒附着至宿主细胞的过程中发挥着至关重要的作用。这种相互作用不仅通过网格蛋白依赖性内吞作用诱导病毒粒子内化，而且还促进了大约三分之一的病毒颗粒的另一种网格蛋白和小窝蛋白独立途径。HA是I类病毒融合蛋白，负责通过介导病毒颗粒膜与内体膜的融合来穿透细胞质。内体中的低pH环境会触发HA2的不可逆构象变化，导致融合疏水肽的释放。几个三聚体的协同作用对于形成有能力的融合孔是必要的，这凸显了HA在宿主范围限制和毒力测定中的复杂作用。

Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 1-10 μg/mL for 1%GRBC.

Virus

Sf9 insect cells

C-His

A4D5Q0 (M1-T546)

/

Approximately 59 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.