HAI-1 蛋白, Mouse (Fc)

The HAI-1 protein is a multifaceted inhibitor that potently inhibits HGFAC and protects against serine proteases such as ST14/matriptase and TMPRSS13. Through its BPTI/Kunitz inhibitor 1 domain, HAI-1 cleverly coordinates inhibition, engaging in a delicate molecular dance with HGFAC and TMPRSS13. HAI-1 Protein, Mouse (Fc) is the recombinant mouse-derived HAI-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of HAI-1 Protein, Mouse (Fc) is 414 a.a., with molecular weight of ~73.2 kDa.

HAI-1 是一种多方面的蛋白质，在多种细胞环境中都是有效的抑制剂。值得注意的是，它作为 HGFAC 的强大抑制剂，对这一关键因素发挥调节作用。作为丝氨酸蛋白酶活性的守护者，HAI-1 对 ST14/matriptase 和 TMPRSS13 具有抑制作用，巧妙地利用其 BPTI/Kunitz 抑制剂 1 结构域。复杂的分子芭蕾涉及与 HGFAC 和 TMPRSS13 的直接相互作用，编排微妙的舞蹈，不仅抑制而且促进特定的细胞过程。与 TMPRSS13 的相互作用强调了 HAI-1 在协调这种丝氨酸蛋白酶的磷酸化和细胞定位中的作用。在这些多方面的相互作用中，HAI-1 作为一个多功能的守护者出现，微调细胞环境中蛋白酶活性的微妙平衡。

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 The IC₅₀ value is 0.35 nM.

Mouse

HEK293

C-hFc

Q9R097 (E30-E443)

20732  [NCBI]

ELPSAPAELPGGAACLSRFTSGVPSFVLDTEASVSNGATFLGSPTARRGWDCVRSCCTTQNCNLALVELQPDGGEDAISACFLMNCLYEQNFVCKFAPKEGFINYLTQELYRSYRELRTRGFGGSRIPRIWMGIDLKVQLQKPLVLNEADNTDWHLLQGDSDVRVERKRPEEVELWGLKEGTYLFQLTRTDSDQPEETANLTITVLTAKQTEDYCLASYKVGRCRGSFPRWYYDPKEQICKSFTFGGCLGNKNNYLREEECMLACKDVQGISPKRHHPVCSGSCHATQFRCSNGCCIDGFLECDDTPDCPDGSDEATCEKYTSGFDELQNIHFLSDKGYCAELPDTGFCKENIPRWYYNPFSERCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREGSIPTVGSAE

Approximately 80-95 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.