1. 重组蛋白
  2. Cytokines and Growth Factors
  3. HB-EGF 蛋白, Human (sf9)

HB-EGF 蛋白, Human (sf9)

目录号: HY-P73093
产品使用指南

HB-EGF 蛋白是一种多功能生长因子,通过 EGFR、ERBB2 和 ERBB4 调节,对心脏瓣膜形成和心脏功能至关重要。它促进平滑肌细胞增殖,并可能有助于巨噬细胞介导的细胞增殖。HB-EGF 蛋白, Human (sf9) 是重组的 HB-EGF 蛋白,由 Sf9 insect cells 表达,不带标签。HB-EGF 蛋白, Human (sf9) 全长 86 个氨基酸,分子量约为 ~16.4 kDa。

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描述

HB-EGF protein is a multifunctional growth factor that is regulated through EGFR, ERBB2 and ERBB4 and is critical for cardiac valve formation and cardiac function. It promotes smooth muscle cell proliferation and may contribute to macrophage-mediated cell proliferation. HB-EGF Protein, Human (sf9) is the recombinant human-derived HB-EGF protein, expressed by Sf9 insect cells , with tag free. The total length of HB-EGF Protein, Human (sf9) is 86 a.a., with molecular weight of ~16.4 kDa.

研究背景

HB-EGF蛋白是一种多功能生长因子,通过EGFR、ERBB2和ERBB4发挥其调节作用。HB-EGF 对于心脏瓣膜形成和正常心脏功能至关重要,在促进平滑肌细胞增殖方面发挥着关键作用,并可能有助于巨噬细胞介导的细胞增殖。HB-EGF 对成纤维细胞具有促有丝分裂特性,同时不影响内皮细胞,其独特之处在于它与 EGF 受体/EGFR 的结合比 EGF 具有更大的亲和力,成为平滑肌细胞更有效的有丝分裂原。除了其增殖作用之外,HB-EGF 还充当白喉毒素受体并与 FBLN1 相互作用。HB-EGF 与 EGFR 和 ERBB4 的多方面相互作用强调了其在细胞调节和心血管发育中的核心作用。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q99075 (D63-L148)

基因 ID
蛋白结构
N-term
HB-EGF (D63-L148)
Accession # Q99075
C-term
同用名
Proheparin-binding EGF-like Growth Factor; DTR; DTS; HEGFL; HBEGF
氨基酸序列

MKLLPSVVLKLFLAAVLSALVTGESLERLRRGLAAGTSNPDPPTVSTDQLLPLGGGRDRKVRDLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL

分子量

Approximately 16.4 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HB-EGF Protein, Human (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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