HBP1 蛋白, Human (His)

HBP1 is a transcriptional repressor that regulates target genes by binding specifically to promoter regions, specifically favoring the sequence 5'-TTCATTCATTCA-3'. Its key role in the cell cycle and Wnt pathway involves promoting enhanced binding to the histone H1.0 promoter through the RB1 interaction. HBP1 Protein, Human (His) is the recombinant human-derived HBP1 protein, expressed by E. coli , with N-His labeled tag. The total length of HBP1 Protein, Human (His) is 138 a.a., with molecular weight of ~17 kDa.

HBP1 是一种转录抑制因子，通过特异性结合靶基因的启动子区域来发挥其调节作用。它在控制细胞周期和 Wnt 通路中发挥着关键作用，显示出对序列 5'-TTCATTCATTCA-3' 的偏好。与 RB1 的相互作用增强了其与组蛋白 H1.0 启动子的结合，有助于其调节功能。此外，HBP1 破坏 DNA-TCF4 相互作用并与 SIN3A 的第二个 PAH 重复序列结合。值得注意的是，它与 TCF4 和 RB1 直接相互作用，进一步强调了它参与复杂的监管网络。

Human

E. coli

N-His

O60381 (P208-F345)

26959  [NCBI]

PSTVWHCFLKGTRLCFHKGSNKEWQDVEDFARAEGCDNEEDLQMGIHKGYGSDGLKLLSHEESVSFGESVLKLTFDPGTVEDGLLTVECKLDHPFYVKNKGWSSFYPSLTVVQHGIPCCEVHIGDVCLPPGHPDAINF

Approximately 17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.