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  3. HCFC2 蛋白, Mouse (His)

HCFC2 蛋白, Mouse (His)

目录号: HY-P70906
产品使用指南

HCFC2 蛋白通过结合 KMT2A/MLL1 并参与 MLL1/MLL 复合物,在细胞过程中发挥重要作用。该复合体以 KMT2A/MLL1、ASH2L、RBBP5、DPY30、WDR5、MEN1、HCFC1 和 HCFC2 为特征,在表观遗传调控和基因表达中发挥着关键作用。HCFC2 蛋白, Mouse (His) 是重组的 HCFC2 蛋白,由 E. coli 表达,带有 N-6*His 标签。HCFC2 蛋白, Mouse (His) 全长 486 个氨基酸,分子量约为 ~55.0 kDa。

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描述

HCFC2 protein is critically involved in cellular processes by binding to KMT2A/MLL1 and participating in the MLL1/MLL complex. This complex, characterized by KMT2A/MLL1, ASH2L, RBBP5, DPY30, WDR5, MEN1, HCFC1, and HCFC2, plays a key role in epigenetic regulation and gene expression. HCFC2 Protein, Mouse (His) is the recombinant mouse-derived HCFC2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HCFC2 Protein, Mouse (His) is 486 a.a., with molecular weight of ~55.0 kDa.

研究背景

HCFC2 蛋白通过与 KMT2A/MLL1 结合并作为 MLL1/MLL 复合物的组成部分在细胞过程中发挥关键作用。该复合体由 KMT2A/MLL1、ASH2L、RBBP5、DPY30、WDR5、MEN1、HCFC1 和 HCFC2 组成,参与表观遗传调控和基因表达。此外,HCFC2 与 TASOR 相互作用,表明它与有助于各种细胞功能的分子复合物相关。这些相互作用强调了 HCFC2 在表观遗传调控和染色质修饰的复杂机制中的重要性。

种属

Mouse

表达系统

E. coli

标签

N-6*His

蛋白编号

G5E837 (M1-E486)

基因 ID

67933  [NCBI]

蛋白结构
N-term
6*His
HCFC2 (M1-E486)
Accession # G5E837
C-term
同用名
Host cell factor 2 ; HCF-2; C2 factor; Hcfc2
氨基酸序列

MAAPSLLNWRRVSSFTGPVPRARHGHRAVAIRELMIIFGGGNEGIADELHVYNTVTNQWFLPAVRGDIPPGCAAHGFVCDGTRILVFGGMVEYGRYSNELYELQASRWLWKKVKPQPPPSGFPPCPRLGHSFSLYGNKCYLFGGLANESEDSNNNVPRYLNDFYELELQHGSGVVGWSIPATKGVVPSPRESHTAIIYCKKDSASPKMYVFGGMCGARLDDLWQLDLETMSWSKPETKGTVPLPRSLHTASVIGNKMYIFGGWVPHKGENPETSPHDCEWRCTSSFSYLNLDTAEWTTLVSDSQEDKKNSRPRPRAGHCAVAIGTRLYFWSGRDGYKKALNSQVCCKDLWYLDTEKPPAPSQVQLIKATTNSFHVKWDEVPTVEGYLLQLNTDLTYQATSSDSSAAPSVLGGRMDPHRQGSNSTLHNSVSDTVNSTKTEHTAVRGTSLRSKPDSRAVDSSAALHSPLAPNTSNNSSWVTDMLRKNE

分子量

Approximately 55.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HCFC2 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HCFC2 Protein, Mouse (His)
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HY-P70906
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