1. 重组蛋白
  2. Viral Proteins
  3. HCoV-HKU1 Spike 蛋白 (Q0ZME7, sf9, His)

HCoV-HKU1 Spike 蛋白 (Q0ZME7, sf9, His)

目录号: HY-P77386
产品使用指南

HCoV-HKU1 刺突蛋白通过与宿主受体结合来启动病毒感染,促进病毒颗粒附着在细胞膜上。作为I类病毒融合蛋白,它通过一系列构象状态协调病毒体与细胞膜之间的融合。HCoV-HKU1 Spike 蛋白 (Q0ZME7, sf9, His) 是重组的 HCoV-HKU1 刺突蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

The HCoV-HKU1 spike protein initiates viral infection by binding to host receptors, promoting attachment of viral particles to the cell membrane. As a class I viral fusion protein, it coordinates the fusion between the virion and the cell membrane through a series of conformational states. HCoV-HKU1 Spike Protein (Q0ZME7, sf9, His) is the recombinant Virus-derived HCoV-HKU1 Spike protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

HCoV-HKU1 Spike/S1 蛋白通过与宿主受体相互作用将病毒颗粒附着在细胞膜上,从而启动感染,从而在病毒感染过程中发挥关键作用。作为 I 类病毒融合蛋白,它介导病毒粒子与细胞膜的融合。该蛋白质经历至少三种构象状态:融合前天然状态、发夹前中间状态和融合后发夹状态。在病毒和靶细胞膜之间的融合过程中,卷曲螺旋区域(七肽重复序列)采用发夹三聚体结构,将融合肽定位在紧邻胞外域的C端区域的位置。这种结构重排对于驱动病毒和靶细胞膜的并置和随后的融合至关重要。

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q0ZME7 (A13-P1295)

基因 ID

/

蛋白结构
N-term
HCoV-HKU1 Spike (A13-P1295)
Accession # Q0ZME7
His
C-term
同用名
Human coronAvirus HKU1 (isolate N5) (HCoV-HKU1) Spike Protein (S1+S2, His)
分子量

Approximately 144.4 kDa.

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HCoV-HKU1 Spike Protein (Q0ZME7, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HCoV-HKU1 Spike Protein (Q0ZME7, sf9, His)
目录号:
HY-P77386
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