2. 重组蛋白
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin 蛋白, H11N2 (AAY85533, HEK293, His)

HA/Hemagglutinin 蛋白, H11N2 (AAY85533, HEK293, His)

目录号: HY-P77014
产品使用指南

HA/Hemagglutinin 蛋白是一类病毒融合蛋白。通过介导内吞病毒颗粒的膜与内体膜的融合，负责将病毒渗透到细胞质中。HA/Hemagglutinin 蛋白 结合唾液酸受体，使病毒颗粒附着在细胞上。HA/Hemagglutinin 蛋白是一种同源三聚体，由位于膜近端茎结构域顶部的球状头结构域组成.HA/Hemagglutinin 蛋白, H11N2 (AAY85533, HEK293, His) 是重组的 血凝素/HA 蛋白，由 HEK293 表达，带有 C-His 标签。

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描述

HA/Hemagglutinin Protein is a type of viral fusion protein. It mediates the fusion of the membrane of endocytosed virus particles with the endosomal membrane, responsible for the penetration of the virus into the cytoplasm. HA/Hemagglutinin Protein binds to sialic acid receptors, allowing the virus particles to attach to cells. HA/Hemagglutinin Protein is a homotrimer consisting of a globular head domain located at the top of the stem domain near the membrane. Hemagglutinin/HA Protein, H11N2 (AAY85533, HEK293, His) is the recombinant Virus-derived Hemagglutinin/HA protein, expressed by HEK293 , with C-His labeled tag.
研究背景

HA/Hemagglutinin 蛋白是一类病毒融合蛋白，通过介导内吞病毒颗粒的膜与内体膜的融合，负责将病毒渗透到细胞质中。HA/Hemagglutinin Protein 介导的膜融合发生在体内且依赖体内的PH值，体内的酸性环境会触发 HA/Hemagglutinin Protein 的构象变化，进而诱导病毒-宿主膜融合。HA/Hemagglutinin Protein 结合唾液酸受体，使病毒颗粒附着在细胞上。HA/Hemagglutinin 蛋白是一种同源三聚体，由位于膜近端茎结构域顶部的球状头结构域组成[1][2]。
种属

Virus
表达系统

HEK293
标签

C-His
蛋白编号

AAY85533 (D17-K528)
基因 ID

/
蛋白结构
N-term
HA/Hemagglutinin (D17-K528)
Accession # AAY85533
His
C-term
同用名
Influenza A H11N2 (A/duck/Yangzhou/906/2002) Hemagglutinin / HA Protein (His)
分子量

Approximately 70 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HA/Hemagglutinin Protein, H11N2 (AAY85533, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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