1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, H3N2 ( EPI189220, sf9, His)

HA/Hemagglutinin 蛋白, H3N2 ( EPI189220, sf9, His)

目录号: HY-P75118
产品使用指南

血凝素 HA1 蛋白通过与含唾液酸的受体结合,通过网格蛋白依赖性或网格蛋白和小窝蛋白独立途径诱导病毒颗粒内化,对于病毒颗粒与宿主细胞的附着至关重要。作为 I 类病毒融合蛋白,HA1 决定宿主范围限制和毒力,介导病毒体膜和内体膜之间的融合。HA/Hemagglutinin 蛋白, H3N2 ( EPI189220, sf9, His) 是重组的 HA/血凝素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

The hemagglutinin HA1 protein is essential for attachment of virions to host cells by binding to sialic acid-containing receptors, inducing virion internalization via clathrin-dependent or clathrin- and caveolin-independent pathways. As a class I viral fusion protein, HA1 determines host range restriction and virulence, mediating fusion between the virion membrane and the endosomal membrane. HA/Hemagglutinin Protein, H3N2 ( EPI189220, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

血凝素 HA1 蛋白通过与细胞表面上含唾液酸的受体结合,有助于病毒颗粒附着到宿主细胞上。这种附着通过网格蛋白依赖性内吞作用或网格蛋白和小窝蛋白独立途径触发病毒粒子内化。HA1 在确定宿主范围限制和毒力方面发挥着至关重要的作用,作为 I 类病毒融合蛋白,通过介导内吞病毒颗粒膜与内体膜的融合,促进病毒渗透到细胞质中。内体中的低pH环境会诱导HA2发生不可逆的构象变化,从而导致融合疏水肽的释放。一个有能力的融合孔的形成需要几个三聚体的协同作用,这强调了HA1促进病毒进入和感染的复杂机制。

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

EPI189220.1 (Q17-W530)

基因 ID

/

蛋白结构
N-term
HA/Hemagglutinin (Q17-W530)
Accession # EPI189220.1
His
C-term
同用名
Influenza A H3N2 (A/Victoria/208/2009) Hemagglutinin / HA Protein (sf9, His)
分子量

Approximately 59.2 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, H3N2 ( EPI189220, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, H3N2 ( EPI189220, sf9, His)
目录号:
HY-P75118
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