1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, H6N4 (CAC84244, HEK293, His)

HA/Hemagglutinin 蛋白, H6N4 (CAC84244, HEK293, His)

目录号: HY-P77032
产品使用指南

血凝素/HA 蛋白附着在唾液酸受体上并启动病毒与细胞的结合。这种附着通过网格蛋白依赖性或不依赖性途径促进病毒粒子内化。HA/Hemagglutinin 蛋白, H6N4 (CAC84244, HEK293, His) 是重组的 血凝素/HA 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

The hemagglutinin/HA protein attaches to sialic acid receptors and initiates virus binding to cells. This attachment promotes virion internalization via clathrin-dependent or -independent pathways. Hemagglutinin/HA Protein, H6N4 (CAC84244, HEK293, His) is the recombinant Virus-derived Hemagglutinin/HA protein, expressed by HEK293 , with C-His labeled tag.

研究背景

血凝素/HA 蛋白与细胞表面上含唾液酸的受体结合,导致病毒颗粒附着在细胞上。这种附着可以通过网格蛋白依赖性内吞作用或网格蛋白和小窝蛋白独立途径触发病毒颗粒内化。HA蛋白在决定病毒的宿主范围限制和毒力方面发挥着至关重要的作用。作为I类病毒融合蛋白,它负责通过介导内吞病毒颗粒膜与内体膜之间的融合来促进病毒渗透到细胞质中。在内体的低pH环境下,HA2发生不可逆的构象变化,导致融合疏水肽的释放。需要多个HA三聚体才能形成有能力的融合孔。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

Q8UWX0 (D17-Q529)

基因 ID

/

蛋白结构
N-term
HA/Hemagglutinin (D17-Q529)
Accession # Q8UWX0
His
C-term
同用名
Influenza A H6N4 (A/chicken/Hong Kong/17/1977) Hemagglutinin / HA Protein (His)
分子量

Approximately 70 kDa

纯度

Greater than 88% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, H6N4 (CAC84244, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, H6N4 (CAC84244, HEK293, His)
目录号:
HY-P77032
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