1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA1/Hemagglutinin 蛋白, H4N8 (P19695, HEK293, His)

HA1/Hemagglutinin 蛋白, H4N8 (P19695, HEK293, His)

目录号: HY-P74948
产品使用指南

HA1/血凝素蛋白与细胞表面受体结合,促进病毒附着。它通过内吞作用导致内化。HA1 决定宿主范围和毒力,介导病毒与内体膜的融合。低 pH 值会触发 HA2 构象变化,释放融合肽。多个HA三聚体形成融合孔。HA1/Hemagglutinin 蛋白, H4N8 (P19695, HEK293, His) 是重组的 HA1/血凝素蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

HA1/Hemagglutinin Protein, binding to cell surface receptors, facilitates virus attachment.It leads to internalization via endocytosis.HA1 determines host range and virulence, mediating fusion of virus and endosomal membrane.Low pH triggers HA2 conformational change, releasing fusion peptide.Multiple HA trimers form fusion pore.HA1/Hemagglutinin Protein, H4N8 (P19695, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

研究背景

HA1蛋白,也称为血凝素,与细胞表面上含唾液酸的受体结合,促进病毒颗粒附着到细胞上。这种附着导致约三分之二的病毒颗粒通过网格蛋白依赖性内吞作用内化,约三分之一通过网格蛋白和小窝蛋白独立途径内化。HA1在确定病毒的宿主范围限制和毒力方面也起着至关重要的作用。作为I类病毒融合蛋白,它负责通过介导内吞病毒颗粒膜与内体膜的融合来穿透细胞质。内体中的低pH环境触发HA2的不可逆构象变化,释放融合疏水肽。有能力的融合孔的形成需要多个HA三聚体。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

P19695 (Q17-R343)

基因 ID

/

蛋白结构
N-term
HA1/Hemagglutinin (Q17-R343)
Accession # P19695
His
C-term
同用名
Influenza A H4N8 (A/chicken/Alabama/1/1975) Hemagglutinin Protein (HA1 Subunit) (His)
分子量

Approximately 55 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA1/Hemagglutinin Protein, H4N8 (P19695, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA1/Hemagglutinin Protein, H4N8 (P19695, HEK293, His)
目录号:
HY-P74948
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