Heparinase II 蛋白, P. heparinus (His)

Heparinase II protein is an enzyme that cleaves heparin and heparan sulfate glycosaminoglycans using a beta elimination mechanism. Specifically, it cleaves heparin at the α-D-GlcNp2S6S(1->4) α-L-IdoAp2S site and at the α-D-GlcNp2Ac(or 2S)6OH(1->4)beta-D-GlcAp position Site of cleavage of heparan sulfate. Heparinase II Protein, P. heparinus (His) is the recombinant Heparinase II protein, expressed by E. coli , with N-His labeled tag. The total length of Heparinase II Protein, P. heparinus (His) is 747 a.a., with molecular weight of 66-75 kDa.

肝素酶 II 是一种具有使用 β 消除机制裂解肝素和硫酸乙酰肝素糖胺聚糖的卓越能力的蛋白质。该酶促过程涉及肝素在 α-D-GlcNp2S6S(1->4) α-L-IdoAp2S 处的裂解和硫酸乙酰肝素在 α-D-GlcNp2Ac(或 2S)6OH(1->4)beta-D-GlcAp 处的裂解。通过催化这些特定的裂解反应，肝素酶 II 在这些复杂糖胺聚糖的结构修饰和降解中发挥着至关重要的作用。该酶能够靶向肝素和硫酸乙酰肝素中不同的糖苷键，使其成为研究这些多糖的宝贵工具，有助于我们了解其生物学功能和潜在的治疗应用（参考：未提供具体参考）。

Measured by its ability to liberate oligosaccharides from 3 mg/mL heparin. The specific activity is 808.9 pmol/min/µg that incubate at room temperature in kinetic mode for 5 minutes.

Others

E. coli

N-6*His

C6XZB6/WP_015808224.1 (Q26-R772)

/

QTKADVVWKDVDGVSMPIPPKTHPRLYLREQQVPDLKNRMNDPKLKKVWADMIKMQEDWKPADIPEVKDFRFYFNQKGLTVRVELMALNYLMTKDPKVGREAITSIIDTLETATFKPAGDISRGIGLFMVTGAIVYDWCYDQLKPEEKTRFVKAFVRLAKMLECGYPPVKDKSIVGHASEWMIMRDLLSVGIAIYDEFPEMYNLAAGRFFKEHLVARNWFYPSHNYHQGMSYLNVRFTNDLFALWILDRMGAGNVFNPGQQFILYDAIYKRRPDGQILAGGDVDYSRKKPKYYTMPALLAGSYYKDEYLNYEFLKDPNVEPHCKLFEFLWRDTQLGSRKPDDLPLSRYSGSPFGWMIARTGWGPESVIAEMKVNEYSFLNHQHQDAGAFQIYYKGPLAIDAGSYTGSSGGYNSPHNKNFFKRTIAHNSLLIYDPKETFSSSGYGGSDHTDFAANDGGQRLPGKGWIAPRDLKEMLAGDFRTGKILAQGFGPDNQTPDYTYLKGDITAAYSAKVKEVKRSFLFLNLKDAKVPAAMIVFDKVVASNPDFKKFWLLHSIEQPEIKGNQITIKRTKNGDSGMLVNTALLPDAANSNITSIGGKGKDFWVFGTNYTNDPKPGTDEALERGEWRVEITPKKAAAEDYYLNVIQIADNTQQKLHEVKRIDGDKVVGVQLADRIVTFSKTSETVDRPFGFSVVGKGTFKFVMTDLLPGTWQVLKDGKILYPALSAKGDDGALYFEGTEGTYRFLR

85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.