HGFR 蛋白, Canine (HEK293, His)

The HGFR protein is a receptor tyrosine kinase that transduces signals by binding to hepatocyte growth factor/HGF. It regulates proliferation, scattering, morphogenesis, and cell survival. HGFR Protein, Canine (HEK293, His) is the recombinant canine-derived HGFR protein, expressed by HEK293 , with N-His labeled tag. The total length of HGFR Protein, Canine (HEK293, His) is 911 a.a., with molecular weight of ~35-43 kDa & 80-100 kDa, respectively.

HGFR 蛋白是一种受体酪氨酸激酶，通过与肝细胞生长因子/HGF 配体结合，充当细胞外基质信号的关键转导器。它在调节各种生理过程中发挥着关键作用，包括增殖、散射、形态发生和细胞存活。配体在细胞表面结合后，MET 在其胞内结构域上发生自磷酸化，为下游信号分子创建对接位点。激活后，HGFR 与 PI3 激酶亚基 PIK3R1、PLCG1、SRC、GRB2、STAT3 或适配器 GAB1 相互作用，导致多个信号级联的激活，例如 RAS-ERK、PI3 激酶-AKT 和 PLCgamma-PKC。RAS-ERK 激活与形态发生效应相关，而 PI3K/AKT 协调促生存效应。在胚胎发育中，HGFR 信号传导有助于原肠胚形成、肌肉和神经元前体发育和迁移、血管生成和肾脏形成。在成人中，它参与伤口愈合、器官再生、组织重塑，并促进造血细胞的分化和增殖。此外，在微生物感染的情况下，HGFR充当InlB内单核细胞增生李斯特菌的受体，促进病原体进入细胞。

Immobilized Canine HGFR at 5 μg/mL (100 μL/well) can bind Human HGF. The ED₅₀ for this effect is 43.11 ng/mL.

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  Immobilized Canine HGF R at 5 μg/mL (100 μL/well) can bind Human HGF. The ED₅₀ for this effect is 43.11 ng/mL.

Canine

HEK293

N-His

Q75ZY9 (E25-L935)

403438  [NCBI]

ECKEALVKSEMNVNMKYQLPNFTAETPIQNVVLHKHHIYLGAVNYIYVLNDKDLQKVAEYKTGPVLEHPDCSPCQDCSHKANLSGGVWEDNINMALLVDTYYDDQLISCGSVHRGTCQRHILPPSNIADIQSEVHCMYSSQADEEPSQCPDCVVSALGTKVLISEKDRFINFFVGNTINSSDHPDHSLHSISVRRLKETQDGFKFLTDQSYIDVLPEFRDSYPIKYVHAFESNHFIYFLTVQRETLDAQTFHTRIIRFCSVDSGLHSYMEMPLECILTEKRRKRSTREEVFNILQAAYVSKPGAHLAKQIGANLNDDILYGVFAQSKPDSAEPMNRSAVCAFPIKYVNEFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARNDEYRTEFTTALQRVDLFMGQFNQVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGLSTPHVNFRLDSHPVSPEAIVEHPLNQNGYTLVVTGKKITRIPLNGLGCEHFQSCSQCLSAPPFVQCGWCHDRCVHLEECPTGAWTQEVCLPAIYEVFPTSAPLEGGTVLTVCGWDFGFRRNNKFDLKKTKVFLGNESCTLTLSESTTNMLKCTVGPAVNEHFNISIIISNGRGTAQYSTFSYVDPIITSISPSYGPKNGGTLLTLTGKYLNSGNSRHISMGGKTCTLKSVSDSILECYTPAQATATEFPIKLKIDLANREMNSFSYQEDPIVYAIHPTKSFISGGSTITAVGKNLNSVSVLRMVIDVHETRRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGIHSKYFDLIYVHNPVFKPFEKPVMISIGNENVLEIKGNDIDPEAVKGEVLKVGNKSCETIYSDSKAVLCKVPNDLLKLNNELNIEWKQAVSSTVLGKVIVQPDQNFTGL

Approximately 35-43 kDa & 80-100 kDa. The protein is a disulfide-linked heterodimer composed of proteolytically cleaved α (32 kDa) and β subunits (120 kDa).

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.