Histone H4 蛋白, Human/Xenopus laevis

Histone H4 proteins are core components of nucleosomes, which compact DNA into chromatin and regulate DNA accessibility during cellular processes. Histones, including H4, play critical roles in transcriptional regulation, DNA repair, replication, and chromosome stability. Histone H4 Protein, Human/Xenopus laevis is the recombinant Xenopus laevis-derived Histone H4 protein, expressed by E. coli , with tag free. The total length of Histone H4 Protein, Human/Xenopus laevis is 102 a.a., with molecular weight of ~11.2 kDa.

组蛋白 H4 蛋白是核小体的核心成分，核小体是染色质结构中负责包裹和压缩 DNA 的基本单位，从而限制了其对依赖 DNA 模板的细胞机器的可及性。包括 H4 在内的组蛋白在转录调节、DNA 修复、DNA 复制和染色体稳定性维持等重要细胞过程中发挥着核心作用。DNA 可及性的复杂调控涉及一系列复杂的翻译后修饰（统称为组蛋白密码）和动态核小体重塑。核小体结构包含一个组蛋白八聚体，每个八聚体含有两个 H2A、H2B、H3 和 H4 分子，组织成一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体。这个八聚体包裹着大约 147 个碱基对的 DNA。此外，组蛋白 H4 参与与 DNJC9、MCM2 和组蛋白 H3.3-H4 二聚体的共伴侣复合物，直接与复合物内的 DNJC9 相互作用。

Xenopus laevis

E. coli

Tag Free

P62805 (S2-G103)

121504  [NCBI]

SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Approximately 11.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of ddH₂O, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.