HMGB3/HMG-3 蛋白, Human (HEK293, His)

The multifunctional HMGB3/HMG-3 protein has multiple cellular functions and exhibits redox-sensitive behavior. It is associated with chromatin and exhibits a preference for non-canonical DNA structures, particularly single-stranded DNA. HMGB3/HMG-3 Protein, Human (HEK293, His) is the recombinant human-derived HMGB3/HMG-3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of HMGB3/HMG-3 Protein, Human (HEK293, His) is 200 a.a., with molecular weight of ~26.0 kDa.

HMGB3/HMG-3 是一种多功能蛋白质，在细胞区室中具有多种功能，表现出氧化还原敏感行为。它与染色质结合，表现出对非规范 DNA 结构的偏好，尤其是单链 DNA，并具有弯曲 DNA 的能力，通过循环增强灵活性。这一特性提供了影响各种基因启动子的机制。HMGB3/HMG-3 被认为参与对核酸的先天免疫反应，可能充当细胞质混杂免疫原性 DNA/RNA 传感器。值得注意的是，它对 B 细胞和骨髓细胞分化产生负调节作用，通过潜在影响自我更新和分化之间的平衡，在造血干细胞中发挥着至关重要的作用。此外，HMGB3/HMG-3 与经典 Wnt 信号传导的负调节有关，进一步强调了其在细胞过程中的多方面作用。

Human

HEK293

C-6*His

O15347 (M1-E200)

3149  [NCBI]

MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSGKEKSKFDEMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLNDSEKQPYITKAAKLKEKYEKDVADYKSKGKFDGAKGPAKVARKKVEEEDEEEEEEEEEEEEEEDE

Approximately 26.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 8% Trehalose, 2% Mannitol, 0.05% Tween 80, 2 mM EDTA, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.