1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. HRAS 蛋白, Human (sf9, His)

HRAS 蛋白, Human (sf9, His)

目录号: HY-P73919
COA 产品使用指南

HRAS 蛋白是启动 Ras 蛋白信号转导的关键参与者,可在分子水平上促进 Ras 信号级联的激活。研究证实,在 HRAS 影响下,Ras 蛋白表现出 GDP/GTP 结合和内在 GTP 酶活性。这些相互作用强调了 HRAS 在协调信号转导动力学中的不可或缺的作用,强调了其在细胞通信和调节中的重要性。HRAS 蛋白, Human (sf9, His) 是重组的 HRAS 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
5 μg ¥780 In-stock
10 μg ¥1250 In-stock
20 μg ¥2000 In-stock
50 μg ¥3800 In-stock
100 μg   询价  

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

HRAS Protein, a key player in initiating Ras protein signal transduction, facilitates the activation of Ras signaling cascades at the molecular level. Under HRAS influence, Ras proteins exhibit GDP/GTP binding and intrinsic GTPase activity, as confirmed by studies. These interactions underscore HRAS's integral role in orchestrating signal transduction dynamics, emphasizing its significance in cellular communication and regulation. HRAS Protein, Human (sf9, His) is the recombinant human-derived HRAS protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

HRAS 蛋白在 Ras 蛋白信号转导(细胞信号转导的关键过程)启动中发挥着关键作用。HRAS 在分子水平上发挥作用,促进 Ras 信号级联的激活。Ras 蛋白在 HRAS 的影响下,表现出结合 GDP/GTP 的能力,并表现出内在的 GTP 酶活性,正如各种研究所证实的。这些分子相互作用强调了 HRAS 在协调信号转导途径动态方面的不可或缺的作用,揭示了其在细胞通讯和调节中的重要性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P01112-1 (M1-C186)

基因 ID
蛋白结构
N-term
HRAS (M1-C186)
Accession # P01112-1
His
C-term
同用名
GTPase Hras; Ha-Ras; HRAS; HRAS1
氨基酸序列

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKC

分子量

Approximately 23 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 10% gly.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

HRAS Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HRAS Protein, Human (sf9, His)
目录号:
HY-P73919
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