HTRA1 蛋白, Human (His)

HTRA1 is a serine protease that targets a variety of proteins, including extracellular matrix proteins such as fibronectin. It induces synovial cells to upregulate MMP1 and MMP3, suggesting a role in extracellular matrix remodeling. HTRA1 Protein, Human (His) is the recombinant human-derived HTRA1 protein, expressed by E. coli , with C-His labeled tag. The total length of HTRA1 Protein, Human (His) is 458 a.a., with molecular weight of 38-40 kDa.

HTRA1 是一种丝氨酸蛋白酶，具有多种靶标，特别是包括纤连蛋白等细胞外基质蛋白。HTRA1 产生的纤连蛋白片段进一步刺激滑膜细胞上调 MMP1 和 MMP3 的产生，表明其在细胞外基质重塑中发挥作用。此外，HTRA1 具有降解蛋白聚糖（如聚集蛋白聚糖、核心蛋白聚糖和纤维调节蛋白）的能力，可能释放可溶性 FGF-糖胺聚糖复合物，从而增强细胞外空间中的 FGF 信号传导。这种多方面的酶通过裂解 IGF 结合蛋白来调节胰岛素样生长因子 (IGF) 的可用性，并抑制 TGF-β 家族成员介导的信号传导，影响各种生理过程，包括视网膜血管生成以及发育过程中的神经元存活和成熟。此外，在细胞内，HTRA1 降解 TSC2，导致下游靶标的激活。在底物存在下同源三聚体和潜在的高阶多聚体的形成凸显了 HTRA1 的复杂调节机制，它还与 BMP4、TGFB1、TGFB2、激活素 A 和 GDF5 等 TGF-β 家族成员相互作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-His

Q92743 (Q23-P480)

5654  [NCBI]

38-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22μm filtered solution of 50 mM Tris, 500 mM NaCl, pH 8.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.