Hydroxyacid oxidase 1 蛋白, Mouse (His)

Hydroxy acid oxidase 1 (HAO1) is a multifunctional (S)-2-hydroxyacid oxidase with a strong preference for glycolate oxidation. This enzymatic process produces glyoxylic acid, an important precursor for the synthesis of glycine by alanine glyoxylate aminotransferase, which prevents glyoxylic acid accumulation and kidney stone formation. Hydroxyacid oxidase 1 Protein, Mouse (His) is the recombinant mouse-derived Hydroxyacid oxidase 1 protein, expressed by E. coli , with N-His labeled tag.

羟基酸氧化酶 1 (HAO1) 是一种多功能 (S)-2-羟基酸氧化酶，具有广泛的底物特异性，对乙醇酸氧化表现出明显的偏好。这种酶活性导致乙醛酸的产生，乙醛酸是丙氨酸-乙醛酸转氨酶过氧化物酶体合成甘氨酸所必需的关键前体。该途径在乙醛酸解毒、防止其积累和肾结石的潜在形成方面发挥着关键作用。HAO1 进一步显示出催化乙醛酸和长链羟基酸（例如 2-羟基十六酸和 2-羟基辛酸）氧化的能力。虽然人工电子受体 2,6-二氯苯酚靛酚 (DCIP) 在体外具有活性，但据信 O2 充当生理电子受体，导致 H2O2 的产生。HAO1 的多方面酶功能强调了其在各种代谢途径中的重要性。

Mouse

E. coli

N-His

Q9WU19 (M1-I370)

15112  [NCBI]

MLPRLVCISDYEQHVRSVLQKSVYDYYRSGANDQETLADNIQAFSRWKLYPRMLRNVADIDLSTSVLGQRVSMPICVGATAMQCMAHVDGELATVRACQTMGTGMMLSSWATSSIEEVAEAGPEALRWMQLYIYKDREISRQIVKRAEKQGYKAIFVTVDTPYLGNRIDDVRNRFKLPPQLRMKNFETNDLAFSPKGNFGDNSGLAEYVAQAIDPSLSWDDITWLRRLTSLPIVVKGILRGDDAKEAVKHGVDGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIIWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI

Approximately 45.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.