IA2 蛋白, Human (293a.a, N-His)

The IA2 protein is critical in the vesicle-mediated secretion process and is essential for the accumulation of secretory vesicles in the hippocampus, pituitary gland, and pancreatic islets. IA2 is critical for insulin-related functions, ensuring normal levels of insulin-containing vesicles, preventing degradation and promoting glucose-stimulated insulin secretion. IA2 Protein, Human (293a.a, N-His) is the recombinant human-derived IA2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IA2 Protein, Human (293a.a, N-His) is 293 a.a., with molecular weight of ~35 kDa.

IA2 蛋白在囊泡介导的分泌过程中发挥着关键作用，其基本功能遍及各种组织。它是海马体、垂体和胰岛等关键区域分泌囊泡正常积累所必需的。IA2 对于胰岛素相关过程尤其重要，它对于正常水平的含胰岛素囊泡的积累、防止其降解并有助于响应葡萄糖刺激而分泌胰岛素是必需的。IA2 的影响还延伸到大脑中的神经递质调节，这是去甲肾上腺素、多巴胺和血清素正常积累所必需的。有趣的是，在女性中，IA2 对于黄体生成素 (LH) 和卵泡刺激素 (FSH) 等垂体激素的正常积累和分泌是不可或缺的。此外，IA2 在维持肾素表达和释放的正常水平方面发挥作用。尽管缺乏内在酶活性，IA2 可以通过二聚化调节催化活性蛋白酪氨酸磷酸酶，例如 PTPRA。IA2 复杂的动力学在其与 ICA512-TMF 的相互作用中得到进一步强调，它调节胰岛素分泌颗粒的动力学和胞吐作用，提出了依赖于 UTRN 并受 ICA512-CFF 二聚化调节的肌动蛋白细胞骨架的束缚机制。

Human

E. coli

N-6*His

Q16849-1 (M687-Q979)

5798  [NCBI]

MDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

Approximately 35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.