ICAM-1/CD54 蛋白, Human (HEK293, His)

The ICAM-1/CD54 protein plays a crucial role in cell interactions, acting as a ligand for the leukocyte adhesion protein LFA-1. It promotes leukocyte transendothelial migration by promoting the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. ICAM-1/CD54 Protein, Human (HEK293, His) is the recombinant human-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-6*His labeled tag.

ICAM-1/CD54 蛋白在细胞相互作用中发挥着重要作用，充当白细胞粘附蛋白 LFA-1（整合素 α-L/β-2）的配体。特别值得注意的是它在白细胞跨内皮迁移过程中的作用，其中与 ICAM-1 的结合通过激活 ARHGEF26/SGEF 和 RHOG 促进内皮顶杯的组装。在微生物感染的情况下，ICAM-1 还充当主要受体组鼻病毒 AB 衣壳蛋白的受体，突显其在免疫反应和病原体识别中的多功能参与。这种双重功能强调了 ICAM-1 在介导对免疫调节和宿主防御至关重要的多种细胞过程中的重要性。

Human

HEK293

C-6*His

P05362 (Q28-E480)

3383  [NCBI]

QTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYE

Approximately 88 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.