ICAM-5 蛋白, Mouse (HEK293, His)

ICAM-5 protein, acting as a ligand for LFA-1, plays a crucial role in cell adhesion, specifically facilitating leukocyte binding to target cells. This interaction is integral to immune responses and inflammation, influencing immune cell recruitment and system modulation. ICAM-5's specificity for LFA-1 underscores its involvement in regulating leukocyte adhesion, a fundamental aspect of immune cell behavior and system functionality. ICAM-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-5 protein, expressed by HEK293 , with C-10*His labeled tag and P47R, , , , mutation. The total length of ICAM-5 Protein, Mouse (HEK293, His) is 798 a.a., .

ICAM-5 蛋白作为白细胞粘附蛋白 LFA-1（整合素 α-L/β-2）的配体具有重要意义。这种相互作用强调了 ICAM-5 在介导细胞粘附过程中的关键作用，特别是促进白细胞与靶细胞的结合。ICAM-5 和 LFA-1 之间的结合是免疫反应和炎症反应不可或缺的一部分，其中适当的细胞间相互作用对于免疫细胞招募和免疫系统功能调节至关重要。ICAM-5 对 LFA-1 的特异性凸显了其参与调节白细胞粘附，这是免疫细胞行为和免疫系统功能的一个基本方面。

Measured by its ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5×10⁴ cells/well are added to ICAM-5 coated plates (25 μg/mL,100 μL/well), 46.215% will adhere after 1 hour at 37°C.

Mouse

HEK293

C-10*His

Q60625 (L31-R828, P47R)

15898

LEPFWADLQPRVALVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELVPLPSWQPVGENFTLSCRVPGAGPRASLTLTLLRGGQELIRRSFVGEPPRARGAMLTARVLARREDHRVNFSCLAELDLRPHGLGLFANSSAPRQLRTFAMPPHSPSLIAPRVLEVDSERPVTCTLDGLFPAPEAGVYLSLGDQRLNPNVTLDGDSLVATATATASAEQEGTKQLMCVVTLGGETRETQENLTVYSFPTPLLTLSEPEAPEGKMVTISCWAGARALVTLEGIPAAVPGQPAELQLNVTKNDDKRGFFCDAALDVDGETLRKNQSSELRVLYAPRLDDLDCPRSWTWPEGPEQTLHCEARGNPEPSVHCARPEGGAVLALGLLGPVTRALAGTYRCTAVNGQGQAVKDVTLTVEYAPALDSVGCPEHITWLEGTEASLSCVAPGVPPPSVSCVRSGKEEVMEGPLRVAREHAGTYRCEAINARGSAAKNVAVTVEYGPSFEELGCPSNWTWVEGSGKLFSCEVDGKPEPRVECVGSEGASEGIVLPLVSSNSGPRNSMTPGNLSPGIYLCNATNRHGSTVKTVVVSAESPPQMDESSCPSHQTWLEGAEATALACSARGRPSPRVHCSREGAARLERLQVSREDAGTYRCVATNAHGTDSRTVTVGVEYRPVVAELAASPPSVRPGGNFTLTCRAEAWPPAQISWRAPPGALNLGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVR

Approximately 130-140 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-Citrate, 300 mM NaCl, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.