IDH1 蛋白, Human (HEK293, C-His)

The IDH1 protein catalyzes the oxidative decarboxylation of isocitrate to generate 2-oxoglutarate, which is utilized by enzymes such as phytoacyl-CoA dioxygenase. IDH1 Protein, Human (HEK293, C-His) is the recombinant human-derived IDH1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of IDH1 Protein, Human (HEK293, C-His) is 414 a.a., with molecular weight of ~48 kDa.

IDH1 蛋白是一种关键酶，可催化 NADP(+) 依赖性异柠檬酸（D-苏式异柠檬酸）氧化脱羧为 2-酮戊二酸（2-酮戊二酸），这是细胞代谢中的重要步骤，也是其他酶（如作为植酰辅酶A双加氧酶。IDH1 的酶活性对于 NADPH 的生成至关重要，NADPH 是参与各种生物合成途径的关键辅助因子。此外，IDH1 作为角膜上皮晶状体蛋白可能具有独特的作用，可能有助于维持角膜上皮透明度。这些多方面的功能凸显了 IDH1 在基本细胞过程中的重要性，并表明它参与维持特定的组织特征。

Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is 2941 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-6*His

O75874 (M1-L414)

3417

MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL

Approximately 48 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.