IFN-alpha 13/IFNA13 蛋白, Mouse (P.pastoris, His)

IFN-alpha 13 (IFNA13), belongs to type I interferon family, is produced by macrophages with antiviral activities^[1]. IFN-alpha 13/IFNA13 Protein, Mouse (P.pastoris, His) contains 166 a.a. (C24-E189), produced in P.pastoris yeast cells with a C-terminal His-tag.

IFN-α 13 (IFNA13；IFN-α13) 由巨噬细胞产生，属于 α/β 干扰素 (IFN) 家族，这是病毒感染诱导的细胞因子家族，主要参与细胞的抗病毒防御 ^[1].干扰素（IFN）最初被认为是一种在体外“干扰”病毒复制的物质。IFN-α/β及相关分子被归类为I型干扰素，其他两类分别为II型干扰素（IFN-γ）和III型干扰素（IFN-λ）^[2]。
干扰素刺激两种酶的产生：蛋白激酶和寡腺苷酸合成酶。干扰素 α (IFNa) 在多种癌症中显示出显着的生物活性，特别是血液系统恶性肿瘤，例如毛细胞白血病和慢性粒细胞白血病^[3]。
IFN-α13 对多种癌症具有酸稳定性抗病毒活性泰勒病毒、门戈病毒和水泡性口炎病毒。首先，它是组成型转录，独立于病毒感染和干扰素调节因子7诱导。其次，它包含两个 N-糖基化位点，与包含一个或不包含一个 N-糖基化位点的其他鼠科 IFN-α 亚型不同^[4]。至于IFN在动物模型中的广泛使用，人IFNA13蛋白的氨基酸序列与小鼠有很大差异(64.55%)

Mouse

P. pastoris

C-His

Q80SU4 (C24-E189)

230396  [NCBI]

Approximately 20.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Kumagai Y, et al. Alveolar macrophages are the primary interferon-alpha producer in pulmonary infection with RNA viruses. Immunity. 2007 Aug;27(2):240-52.  [Content Brief]

  [2]. Zhang SY, et al. Inborn errors of interferon (IFN)-mediated immunity in humans: insights into the respective roles of IFN-alpha/beta, IFN-gamma, and IFN-lambda in host defense. Immunol Rev. 2008 Dec;226:29-40.  [Content Brief]

  [3]. Raj NB, et al. Identification of a novel virus-responsive sequence in the promoter of murine interferon-alpha genes. J Biol Chem. 1991 Jun 15;266(17):11360-5.  [Content Brief]

  [4]. van Pesch V, et al. Characterization of interferon-alpha 13, a novel constitutive murine interferon-alpha subtype. J Biol Chem. 2003 Nov 21;278(47):46321-8.  [Content Brief]