IFN-gamma 蛋白, Feline

IFN-gamma (Interferon-gamma) is a type II interferon produced by immune cells such as T cells and NK cells, which can significantly activate antibacterial, antiviral and anti-tumor responses. Its main JAK-STAT signaling pathway is triggered by interaction with the receptor IFNGR1, affecting gene regulation. IFN-gamma Protein, Feline is the recombinant IFN-gamma protein, expressed by E. coli , with tag free. The total length of IFN-gamma Protein, Feline is 144 a.a., with molecular weight of ~17.1 kDa.

IFN-gamma（干扰素-gamma）是一种由 T 细胞和 NK 细胞等免疫细胞产生的 II 型干扰素，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中发挥关键作用。其主要信号传导在与其受体 IFNGR1 相互作用后通过 JAK-STAT 途径发生，从而影响基因调控。IFN-γ 结合后，IFNGR1 胞内结构域打开，促进下游信号成分（包括 JAK2、JAK1 和 STAT1）的关联，导致 STAT1 激活、核转位和 IFNG 调节基因的转录。许多诱导基因是转录因子，例如 IRF1，能够进一步驱动后续转录波的调节。IFN-γ 通过诱导免疫蛋白酶体亚基替换催化蛋白酶体亚基，从而在 I 类抗原呈递途径中发挥作用，从而增加 I 类 MHC 负载的肽的数量、质量和库。它通过诱导激活剂 PA28 的表达来提高肽生成的效率，PA28 与蛋白酶体结合并改变其蛋白水解切割偏好。此外，IFN-γ 通过促进组织蛋白酶 B/CTSB、H/CTSH 和 L/CTSL 等几种关键分子的表达，上调细胞表面的 MHC II 复合物，从而促进免疫反应。IFN-γ 参与造血干细胞在发育过程中和稳态条件下的调节，影响其发育、静止和分化。IFN-γ 作为同二聚体存在，通过其胞外结构域与 IFNGR1 相互作用，这是促进 IFNGR1 二聚化的关键相互作用，协调其在免疫反应和造血中的多样化和关键功能。

1.Measured in an anti-viral assay using CRFK feline kidney epithelial cells infected with vesicular stomatitis virus (VSV). The ED₅₀ for this effect is 0.15-0.9 ng/mL.
2.Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED₅₀ for this effect is 0.1612 ng/mL, corresponding to a specificactivity is 6.203×10⁶ Unit/mg.

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  Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED₅₀ for this effect is 0.1612 ng/mL, corresponding to a specificactivity is 6.203×10⁶ Unit/mg.

Others

E. coli

Tag Free

P46402 (Q24-K167)

493965  [NCBI]

QAMFFKEIEELKGYFNASNPDVADGGSLFVDILKNWKEESDKTIIQSQIVSFYLKMFENLKDDDQRIQRSMDTIKEDMLDKLLNTSSSKRDDFLKLIQIPVNDLQVQRKAINELFKVMNDLSPRSNLRKRKRSQNLFRGRRASK

Approximately 17.1 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile 50 mM Tris-HCL, 300 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 50 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.