IFN-gamma 蛋白, Human (HEK293, His-Avi)

IFN-gamma Protein is a dimeric soluble cytokine. It exerts antibacterial, antiviral and antitumor effects through JAK-STAT, mTOR, MAPK and PI3K/AKT signaling pathways. IFN-gamma Protein, Human (HEK293, His-Avi) is the recombinant human-derived IFN-gamma protein, expressed by HEK293 , with C-His, C-Avi labeled tag. The total length of IFN-gamma Protein, Human (HEK293, His-Avi) is 138 a.a., with molecular weight of 20-35 kDa.

IFN-gamma 是一种二聚体可溶性细胞因子，是II 型干扰素中唯一的成员。IFN-gamma 由免疫细胞T 细胞和NK 细胞产生，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中发挥重要作用。IFN-gamma 通过JAK-STAT 通路与其受体IFNGR1 相互作用后影响基因调控，也可触发mTOR、MAPK 和PI3K/AKT信号通路。IFN-gamma 通过诱导催化蛋白酶体亚基替换为免疫蛋白酶体亚基，在I 类抗原递呈途径中发挥作用。IFN-gamma 通过促进组织蛋白酶B/CTSB、H/CTSH 和L/CTSL 等几个关键分子的表达，上调细胞表面的MHC II 复合物。IFN-gamma 通过影响造血干细胞的发育、静止和分化，参与造血干细胞在发育和稳态条件下的调控。

Immobilized Human IFN gamma at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Human IFNGR1 hFc with the EC₅₀ of 7.9 ng/mL determined by ELISA.

Human

HEK293

C-His;C-Avi

P01579 (Q24-G161)

3458  [NCBI]

20-35 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5-8 % trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.