IFN-gamma 蛋白, Mouse (HEK293, Fc)

IFN-gamma is a type II interferon family cytokine that participates in antiviral, antibacterial, and antitumor responses. IFN-gamma Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived IFN-gamma protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IFN-gamma Protein, Mouse (HEK293, Fc) is 133 a.a., with molecular weight of ~55 kDa.

IFN-gamma 由免疫细胞如 t 细胞和 NK 细胞产生，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中起关键作用^[1][2][5][6]。FN-gamma 通过影响造血干细胞的发育、静止和分化，参与造血干细胞在发育和稳态条件下的调控^[3][4]。IFN-gamma 通过调节 Fas/FasL、TNF-相关凋亡-诱导配体 (TRAIL)、caspase-8、-3、-7 和 -1、survivin和 Bim 的表达来增加癌细胞对外在和内在凋亡途径的易感性^[5]。IFN-gamma 主要通过 JAK-STAT 通路与其受体 IFNGR1 相互作用，影响基因调控。IFN-gamma 与受体结合后，IFNGR1 的胞内结构域打开，允许下游信号元件 JAK2、JAK1 和 STAT1 结合，导致 STAT1 激活、核易位和 IFN-gamma 调控基因转录^[6]。IFN-gamma 通过诱导RNA活化蛋白激酶R (PKR)和腺苷脱氨酶RNA特异性-1 (ADAR-1)来实现抗病毒作用^[6]。IFN-gamma 能抑制 TH1 细胞产生 IL-4 并且维持 T-bet 的持续表达^[7]。IFN-gamma 作为细胞介导的免疫中心效应因子，可以通过同源t细胞相互作用增强抗原识别，通过抗原呈递细胞 (APCs) 放大抗原呈递，增加活性氧 (ROS) 和活性氮中间体 (RNIs) 的产生，诱导抗病毒反应^[8]。

1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED₅₀ for this effect is 2-15 ng/mL.
2. Measured by its ability to bind with recombinant mouse IFNGR1-His in a functional ELISA.

Mouse

HEK293

C-hFc

P01580 (H23-C155)

15978  [NCBI]

Approximately 55 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.