Ig kappa 蛋白, Human (HEK293, His)

Ig kappa protein is the constant region of an immunoglobulin light chain and is part of a membrane-bound or secreted glycoprotein produced by B lymphocytes. Ig kappa Protein, Human (HEK293, His) is the recombinant human-derived Ig kappa protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Ig kappa Protein, Human (HEK293, His) is 106 a.a., with molecular weight of ~14 kDa.

免疫球蛋白轻链恒定区（以 Ig kappa 蛋白为例）在抗体（也称为免疫球蛋白）的结构和功能中起着至关重要的作用。这些膜结合或分泌的糖蛋白由 B 淋巴细胞产生，在体液免疫识别阶段充当受体。结合特定抗原后，膜结合免疫球蛋白启动 B 淋巴细胞的克隆扩增和分化为分泌免疫球蛋白的浆细胞。分泌的免疫球蛋白反过来协调体液免疫的效应阶段，导致结合抗原的消除。抗原结合位点由一条重链的可变结构域与其相关轻链的可变结构域共同形成，导致每种免疫球蛋白具有两个对特定抗原具有显着亲和力的抗原结合位点。可变结构域经历 V-(D)-J 重排和随后的体细胞超突变，从而在暴露于抗原和选择后允许亲和力成熟。免疫球蛋白的特征是两条相同的重链和两条相同的轻链，通过二硫键错综复杂地连接。

Human

HEK293

C-6*His

P01834 (T2-C107)

3514

TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Approximately 14 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.