IGFBP-3 蛋白, Human (HEK293, His)

IGFBP-3 protein is a part of IGFBP that significantly prolongs IGF half-life and modulates growth effects in cell culture. It inhibits or stimulates IGF-induced cellular responses by changing the kinetics of interaction with cell surface receptors. IGFBP-3 Protein, Human (HEK293, His) is the recombinant human-derived IGFBP-3 protein, expressed by HEK293 , with N-His labeled tag.

IGFBP-3 蛋白是胰岛素样生长因子结合蛋白 (IGFBP) 的成员，在延长胰岛素样生长因子 (IGF) 的半衰期和调节其对细胞培养的生长促进作用方面发挥着至关重要的作用。IGFBP-3 具有抑制或刺激 IGF 诱导的细胞反应的能力，通过改变 IGF 与其各自的细胞表面受体之间的相互作用动力学来发挥其调节影响。此外，IGFBP-3 还表现出由其受体 TMEM219/IGFBP-3R 介导的不依赖于 IGF 的抗增殖和凋亡作用。此外，IGFBP-3 抑制护脑素对胰岛素敏感性的积极作用并促进睾丸生殖细胞凋亡。与 XLKD1 的相互作用以及与 IGF1 或 IGF2 和糖蛋白 (ALS) 形成的三元复合物进一步强调了 IGFBP-3 的多种作用。复杂的相互作用网络，包括与护脑素和 TMEM219 的相互作用，凸显了 IGFBP-3 在调节与生长和凋亡相关的细胞过程和信号通路方面的多方面性质。

1. Measured by its binding ability in a functional ELISA. Immobilized human IGF2 at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC₅₀ of biotinylated Human His-IGFBP3 is ≤18 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized human IGF1 at 10 μg/mL (100 μl/well) can bind biotinylated Human His-IGFBP3, The EC₅₀ of biotinylated Human His-IGFBP3 is ≤24 ng/mL.
3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED₅₀ for this effect is typically 0.05-0.2 μg/mL in the presence of 14 ng/mL human IGFII.

Human

HEK293

N-His

P17936-1/NP_000589.2 (G28-K291)

3486  [NCBI]

40-45 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.