IGFBP-4 蛋白, Mouse (HEK293, His)

IGFBP-4 protein modulates IGFs' activity and can either inhibit or stimulate their growth-promoting effects. It alters the interaction between IGFs and their receptors, with a preference for binding to IGF2. This highlights the nuanced role of IGFBP-4 in fine-tuning IGF-mediated cellular responses. IGFBP-4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-4 protein, expressed by HEK293 , with C-His labeled tag.

IGFBP-4 蛋白通过延长胰岛素样生长因子 (IGF) 的半衰期，在调节其活性方面发挥着关键作用。IGFBP-4 在细胞培养中表现出双重调节影响，可以抑制或刺激 IGF 的生长促进作用，强调其对细胞过程的多功能影响。此外，IGFBP-4 参与改变 IGF 与其细胞表面受体之间的相互作用。值得注意的是，与 IGF1 相比，它更倾向于与 IGF2 结合，表明对特定 IGF 亚型具有特异性亲和力。这种选择性结合特征强调了 IGFBP-4 在微调与 IGF 介导的细胞反应相关的信号通路方面的作用的微妙本质。

Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. 1988, Cancer Research 48: 4083.) . The ED₅₀ for this effect is typically 0.1-0.6 μg/mL.

Mouse

HEK293

C-His

P47879 (D22-E254)

16010  [NCBI]

MLPFGLVAALLLAAGPRPSLGDEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGMRCYPPRGVEKPLRTLMHGQGVCTELSEIEAIQESLQTSDKDESEHPNNSFNPCSAHDHRCLQKHMAKIRDRSKMKIVGTPREEPRPVPQGSCQSELHRALERLAASQSRTHEDLFIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFQE

Approximately 38 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.