IGFBP-5 蛋白, Cynomolgus (HEK293, His)

IGFBP-5 protein modulates IGFs, extending their life span and regulating their growth effects. It inhibits or stimulates IGFs, showing its dual function. By altering IGFs' interaction with cell receptors, IGFBP-5 fine-tunes growth-associated signaling pathways, governing cellular responses. This interplay reveals IGFBP-5's multifaceted role in cellular growth and proliferation. IGFBP-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IGFBP-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-5 Protein, Cynomolgus (HEK293, His) is 252 a.a., with molecular weight of ~35 kDa.

IGFBP-5 蛋白通过延长胰岛素样生长因子 (IGF) 的半衰期，在调节其活性方面发挥着关键作用。在细胞培养中，IGFBP-5 表现出双重调节功能，具有抑制或刺激 IGF 生长促进作用的能力。这种动态影响强调了 IGFBP-5 对细胞过程影响的复杂性。值得注意的是，IGFBP-5通过改变IGF与其细胞表面受体之间的相互作用来实现这些调节作用，从而精细调节与细胞生长和发育相关的信号通路。IGFBP-5 和 IGF 之间微妙的相互作用凸显了控制细胞对生长因子反应的复杂控制机制，揭示了 IGFBP-5 在协调细胞生长和增殖中的多方面作用。

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ for this effect is 1.063 µg/mL in the presence of 14 ng/mL rhIGF-I, corresponding to a specific activity is 940.734 U/mg.

Cynomolgus

HEK293

C-His

G7PLE3 (L21-E272)

101926781  [NCBI]

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Approximately 35 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.