IGFBP-6 蛋白, Rat (HEK293, His)

IGFBP-6 protein modulates IGFs' activity, extending their half-life and exhibiting dual regulatory capacity.It activates the MAPK pathway, induces cell migration, and interacts with PHB2.These diverse functions emphasize IGFBP-6's multifaceted role in cellular responses related to growth regulation, signaling, and migration.IGFBP-6 Protein, Rat (HEK293, His) is the recombinant rat-derived IGFBP-6 protein, expressed by HEK293 , with C-His labeled tag.

IGFBP-6 蛋白通过延长胰岛素样生长因子 (IGF) 的半衰期，在调节其活性方面发挥着至关重要的作用。IGFBP-6 在细胞培养中表现出双重调节能力，可以抑制或刺激 IGF 的生长促进作用，反映了其对细胞过程的多种影响。除了对 IGF 的影响之外，IGFBP-6 还通过激活 MAPK 通路和促进细胞迁移积极参与细胞信号传导。值得注意的是，它通过其 C 端结构域与 PHB2 相互作用，强调其参与复杂的蛋白质-蛋白质相互作用。这些不同的功能凸显了 IGFBP-6 在协调与生长调节、信号传导和迁移相关的细胞反应中的多方面作用。

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ for this effect is 0.1026 µg/mL, corresponding to a specific activity is 9746.5887 U/mg.

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  Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ for this effect is 0.1026 µg/mL, corresponding to a specific activity is 9746.5887 U/mg.

Rat

HEK293

C-6*His

P35572/NP_037236.1 (A26-G226)

25641  [NCBI]

ALAGCPGCGPGVQEEDAGSPADGCAETGGCFRREGQPCGVYIPKCAPGLQCQPRENEETPLRALLIGQGRCQRARGPSEETTKESKPHGGASRPRDRDRQKNPRTSAAPIRPSPVQDGEMGPCRRHLDSVLQQLQTEVFRGGANGLYVPNCDLRGFYRKQQCRSSQGNRRGPCWCVDPMGQPLPVSPDGQGSSQCSARSSG

Approximately 25-30 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.