IGFBP-5 蛋白, Canine (HEK293, Fc)

IGFBP-5 is only one of a family of six IGFBPs. IGFBP-5 is a 29 kDa phosphorylated and glycosylated protein, which is secreted by a wide variety of cell types during different growth conditions. IGFBP-5, the most conserved IGFBP, is the major protein secreted by skeletal muscles. IGFBP-5 Protein, Canine (HEK293, Fc) is the recombinant canine-derived IGFBP-5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IGFBP-5 Protein, Canine (HEK293, Fc) is 252 a.a., with molecular weight of ~40 & 43 & 45 & 65 KDa, respectively.

IGFBP-5 是 6 个 IGFBP 家族中的一个。IGFBP-5 是一种 29 kDa 的磷酸化和糖基化蛋白，由多种类型的细胞在不同的生长条件下分泌。IGFBP-5 是最保守的 IGFBP，是骨骼肌分泌的主要蛋白。IGFBP-5 可与纤维连接蛋白结合并参与胰岛素样生长因子受体信号通路的调节^[1][2][3][4]。

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ this effect is 1.468 µg/mL, corresponding to a specific activity is 681.199 U/mg.

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  Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED₅₀ this effect is 1.468 µg/mL, corresponding to a specific activity is 681.199 U/mg.

Canine

HEK293

C-hFc

F1PEQ5 (L20-E271)

610316  [NCBI]

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQAKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Approximately 40&43&45&65 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.