2. 重组蛋白
  3. Fc Receptors
  4. Immunoglobulin Fc Region
  5. IgG
  6. IgG 蛋白, Rabbit (T185A, N284S, HEK293)

IgG 蛋白, Rabbit (T185A, N284S, HEK293)

目录号: HY-P73904
COA 产品使用指南

IgG 蛋白，显示具有 104-Thr 序列的 D12 同种异型标记和具有 185-Thr 序列的 E14 标记。IgG 蛋白, Rabbit (T185A, N284S, HEK293) 是重组的 IgG 蛋白，由 HEK293 表达，不带标签。IgG 蛋白, Rabbit (T185A, N284S, HEK293) 全长 223 个氨基酸，分子量为 30-38 kDa。

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SPR 检测服务   重组蛋白定制服务

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
5 μg ￥490 In-stock
10 μg ￥840 In-stock
50 μg ￥2350 In-stock
100 μg ￥4000 询价
> 100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

IgG protein, exhibits the D12 allotypic marker with the 104-Thr sequence and the E14 marker with 185-Thr. IgG Protein, Rabbit (T185A, N284S, HEK293) is the recombinant Rabbit-derived IgG protein, expressed by HEK293 , with tag free. The total length of IgG Protein, Rabbit (T185A, N284S, HEK293) is 223 a.a., with molecular weight of 30-38 kDa.
研究背景

IgG 蛋白，显示具有 104-Thr 序列的 D12 同种异型标记和具有 185-Thr 序列的 E14 标记。
生物活性

Immobilized Rabbit IgG, No Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5 ng/mL determined by ELISA.
种属

Rabbit
表达系统

HEK293
标签

Tag Free
蛋白编号

P01870 (S101-K323, T185A, N284S)
基因 ID

100009097  [NCBI]
蛋白结构
N-term
IgG (S101-K323, T185A, N284S)
Accession # P01870
C-term
同用名
Ig gamma chain C region; IgG
氨基酸序列

SKPTCPPPELLGGPSVFIFPPKPKDTLMISRTPEVTCVVVDVSQDDPEVQFTWYINNEQVRTARPPLREQQFNSTIRVVSTLPIAHQDWLRGKEFKCKVHNKALPAPIEKTISKARGQPLEPKVYTMGPPREELSSRSVSLTCMINGFYPSDISVEWEKNGKAEDNYKTTPAVLDSDGSYFLYSKLSVPTSEWQRGDVFTCSVMHEALHNHYTQKSISRSPGK

分子量

30-38 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液
组分

Supplied as 0.22 μm filtered solution in PBS, 150mM Nacl, 10% Glycerol (pH 7.4).
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (210 KB)

产品使用指南 (1213 KB)

IgG Protein, Rabbit (T185A, N284S, HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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目录号：
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