IgG1 蛋白, Human (D239E, L241M, HEK293)

The IgG1 protein is the constant region of the immunoglobulin heavy chain and serves as a receptor during the recognition phase of humoral immunity. It triggers clonal expansion and differentiation of B lymphocytes into immunoglobulin-secreting plasma cells. IgG1 Protein, Human (D239E, L241M, HEK293) is the recombinant human-derived IgG1 protein, expressed by HEK293 , with tag free.

免疫球蛋白重链的恒定区（称为抗体）代表 B 淋巴细胞产生的膜结合或分泌糖蛋白。在体液免疫的识别阶段，这些膜结合的免疫球蛋白充当受体，在结合特定抗原后启动B淋巴细胞的克隆扩增和分化为分泌免疫球蛋白的浆细胞。分泌的免疫球蛋白在体液免疫的效应阶段发挥着至关重要的作用，导致结合抗原的消除。抗原结合位点由一条重链的可变结构域及其相关轻链的可变结构域形成，导致每种免疫球蛋白具有两个对特定抗原具有显着亲和力的抗原结合位点。可变结构域经历 V-(D)-J 重排和随后的体细胞超突变，从而在暴露和选择后实现特定抗原的亲和力成熟。IgG1 蛋白介导单核细胞的效应功能，触发病毒感染细胞的抗体依赖性细胞毒性 (ADCC)。免疫球蛋白由两条相同的重链和两条相同的轻链组成，通过二硫键互连，并与 FCGR1A、FCGR2A 和 FCGR3A 相互作用以介导各种效应功能。

1.Immobilized Human IgG1Fc at 2 μg/mL (100 μl/well) can bind Human CD64-His.The ED₅₀ for this effect is ≤40 ng/mL.
2.Immobilized Human IgG1 at 5 μg/mL (100 μL/well) can bind biotinylated Human CD64. The ED₅₀ for this effect is 1.844 ng/mL.

Human

HEK293

Tag Free

P01857-1 (D104-K330, D239E, L241M)

3500

DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

Approximately 33 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.