IL-1 alpha 蛋白, Human (HEK293, Fc)

IL-1 alpha protein is located in cells and is a key cytokine connecting innate immunity and adaptive immunity. After binding to IL1R1 through IL1RAP, it forms a high-affinity receptor complex, initiates a signaling cascade with adapter molecules such as MYD88, IRAK1 or IRAK4, and activates the NF-kappa-B and MAPK pathways. IL-1 alpha Protein, Human (HEK293, Fc) is the recombinant human-derived IL-1 alpha protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IL-1 alpha Protein, Human (HEK293, Fc) is 159 a.a., with molecular weight of 54 kDa.

白细胞介素-1 α (IL-1 α) 是一种细胞内细胞因子，几乎存在于所有静止的非造血细胞中，在炎症中发挥着关键作用，并且是先天性免疫系统和适应性免疫系统之间的重要纽带。IL-1α 与其受体 IL1R1 结合后，与其辅助蛋白 IL1RAP 一起形成高亲和力的 IL-1 受体复合物。该复合物启动涉及招募接头分子（例如 MYD88、IRAK1 或 IRAK4）的信号级联，随后导致 NF-kappa-B 和三个 MAPK 通路（p38、p42/p44 和 JNK 通路）的激活。在细胞内，IL-1 α 作为警报素，在细胞死亡时、细胞膜破裂后释放到细胞外空间，诱导炎症并向宿主发出对损伤或损害的反应信号。除了在细胞坏死期间释放危险信号之外，IL-1 α 还可以直接感知 DNA 损伤，作为基因毒性应激的信号，而不损害细胞完整性。此外，IL-1 α 与 TMED10、IL1R1 和 S100A13 等蛋白质的相互作用有助于其调节机制，介导易位、分泌和输出过程。

Human

HEK293

C-hFc

P01583 (S113-A271)

3552  [NCBI]

SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA

54 KDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Less than 1 EU/µg as determined by LAL test.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.