IL-16 蛋白, Human

IL-16 Protein, Human is a proinflammatory cytokine containing 130 amino acids.

大肠杆菌中产生的重组人 IL-16。大肠杆菌是含有 130 个氨基酸的单一非糖基化多肽链。Interleukin-16 是 CD8+ 淋巴细胞中产生的促炎细胞因子，被合成为前体蛋白（pro-IL-16）。据推测，IL-16 前体的 C 末端区域被裂解，释放出具有生物活性的 IL-16。IL-16 主要由 CD8⁺ T 淋巴细胞产生和分泌，通过与 CD4 相互作用，对淋巴细胞、单核细胞和嗜酸性粒细胞发挥趋化剂、生长因子和其他活性^[1]。

Measured by its binding ability in a functional ELISA. Immobilized CD4 at 2 μg/mL (100 μL/well) can bind biotinylated IL-16.The ED₅₀ for this effect is 9.388 ng/mL.

Human

E. coli

Tag Free

Q14005-1 (M1203-S1332)

3603  [NCBI]

MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS

18-20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM acetic buffer, pH 5.0, 150 mM NaCl and 0.5 mM DTT or 20 mM PB, 150 mM NaCl, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zhang Y, et al. Processing and activation of pro-interleukin-16 by caspase-3. J Biol Chem. 1998 Jan 9;273(2):1144-9.  [Content Brief]