IL-17B 蛋白, Mouse (sf9, Fc)

IL-17B is a proinflammatory cytokine and stimulates the release of tumour necrosis factor alpha (TNFα) and IL-1β. IL-17B plays an important role in cancer progression, angiogenesis, and inflammatory arthritis^[1][2]. IL-17B Protein, Mouse (sf9, Fc) is a recombinant mouse IL-17B protein with hFc tag at the C-terminus and is expressed in Sf9 insect cells. It consists of 180 amino acids (M1-F180).

白细胞介素-17B (IL-17B) 属于 IL-17 细胞因子家族，是一种促炎细胞因子。IL-17B 在成人胰腺、小肠、胃、脊髓和睾丸中表达。在小鼠整个胚胎中检测到小鼠 IL-17B mRNA^[1][3]。
小鼠 IL-17B 与小鼠 IL-17B 氨基酸序列一致性达 88.33%人类。
IL-17B 作为非共价二聚体糖蛋白分泌。IL-17B 与 IL-17A 具有约 27% 的氨基酸同一性，并刺激 THP-1 单核细胞系中肿瘤坏死因子 α (TNFα) 和 IL-1β 的释放。IL-17B的受体是IL-17RB，属于IL-17受体家族。IL-17B 与 IL-17E 共享 IL-17RB。IL-17B 抑制 IL-17E 与 IL-17RA/IL-17RB 复合物结合，发挥促肿瘤作用和 IL-17E 抗肿瘤活性^[1][2]。
IL-17B 在癌症进展、血管生成和炎症性关节炎中发挥着重要作用^[1]。

mIL-17B (5 ng/mL; 24 h) induces IL-1β expression in 3T3 cells, and induces IL-1β, IL-6, and IL-23 expressions in PECs^[4].

Measured by its ability to induce IL-8 secretion by HepG2 cells. The ED₅₀ for this effect is typically 1.43 μg/mL.

Mouse

Sf9 insect cells

C-hFc

Q9QXT6 (R23-F180)

56069  [NCBI]

Approximately 57 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 100 mM Glycine, 10 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sanders AJ, et al. IL-17B Can Impact on Endothelial Cellular Traits Linked to Tumour Angiogenesis. J Oncol. 2010;2010:817375.  [Content Brief]

  [2]. Bastid J, et al. The Emerging Role of the IL-17B/IL-17RB Pathway in Cancer. Front Immunol. 2020 Apr 21;11:718.  [Content Brief]

  [3]. You Z, et al. Expression of interleukin-17B in mouse embryonic limb buds and regulation by BMP-7 and bFGF. Biochem Biophys Res Commun. 2005 Jan 21;326(3):624-31.  [Content Brief]

  [4]. Yamaguchi Y, et al. IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis. J Immunol. 2007 Nov 15;179(10):7128-36.  [Content Brief]