IL-17F 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

IL-17F is an inflammatory homodimeric cytokine that induces many proinflammatory cytokines and chemokines. IL-17F also induces antimicrobial peptides. IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer^[1][2]. IL-17F Protein, Mouse (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant mouse IL-17F protein with His and Avi tags at the C-terminus and is expressed in HEK293 cells. It consists of 133 amino acids (R29-A161).

白细胞介素-17F (IL-17F) 属于 IL-17 细胞因子家族。IL-17F 在活化的 CD4 T 细胞、活化的单核细胞、嗜碱性粒细胞和肥大细胞中表达。IL-17F 可由分化的 TH17 细胞、固有层 T 细胞、记忆 CD4⁺ T 细胞、γδ T 细胞和 NKT 细胞^[1]产生。
小鼠IL-17F与人氨基酸序列同一性为55.90%，与大鼠氨基酸序列同一性为86.34%。
IL-17F是一种炎症细胞因子，可诱导多种促炎细胞因子和趋化因子，包括TGF-β、IL-2、ICAM1、GM-CSF、CCL2、CCL7、TSLP、MMP13、IL-6和CXCL1。IL-17F还与IL-22一起诱导抗菌肽，包括hBD-2、S100A7、S100A8和S100A9，并且可以与人嗜酸性粒细胞中的IL-23协同作用，促进IL-1β和IL-6的产生。IL-17F 是同型二聚体细胞因子。IL-17F 与 IL-17A 最相似（50% 同源性），并且可以作为 IL-17AF 异二聚体产生。IL-17A、IL-17F和IL-17A/F使用相同的受体复合物：IL-17RA和IL-17RC异二聚体。它们触发性质相似的信号通路，并且 IL-17F 表现出最低的生物活性。IL-17F 对 IL-17RA 亚基的亲和力比 IL-17A 低约 100–1000 倍，并且不与 IL-17A 竞争与 IL-17RA 的结合^[1][2]。
IL-17F 在结肠癌发展中发挥保护作用，可用于自身免疫性疾病、感染和癌症的研究^[1][3][4]。

IL-17F (1 ng/mL; overnight) induces proinflammatory cytokines and chemokines in vitro via IL-17RA, TRAF6, and Act1 in mouse embryonic fibroblasts (MEFs)^[5].

IL-17F (0.5 μg/mouse; i.p.; once) results in the recruitment of neutrophils within 4 h in mice^[5].

Mouse

HEK293

C-Avi;C-His

Q7TNI7-1 (R29-A161)

257630  [NCBI]

30-35 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Chang SH, et al. IL-17F: regulation, signaling and function in inflammation. Cytokine. 2009 Apr;46(1):7-11.  [Content Brief]

  [2]. McGeachy MJ, et al. The IL-17 Family of Cytokines in Health and Disease. Immunity. 2019 Apr 16;50(4):892-906.  [Content Brief]

  [3]. Ferreira N, et al. IL-17A and IL-17F orchestrate macrophages to promote lung cancer. Cell Oncol (Dordr). 2020 Aug;43(4):643-654.  [Content Brief]

  [4]. Tong Z, et al. A protective role by interleukin-17F in colon tumorigenesis. PLoS One. 2012;7(4):e34959.  [Content Brief]

  [5]. Yang XO, et al. Regulation of inflammatory responses by IL-17F. J Exp Med. 2008 May 12;205(5):1063-75.  [Content Brief]