1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-18
  5. IL-18 蛋白, Dog (His)

IL-18 蛋白, Dog (His)

目录号: HY-P71657
产品使用指南

IL-18 蛋白是一种促炎细胞因子,对于上皮屏障修复和免疫调节至关重要,尤其是在 Th1 和 NK 细胞反应中。IL18R1 和 IL18RAP 结合形成信号三元复合物,激活 NF-κ-B 并诱导炎症介质。IL-18 蛋白, Dog (His) 是重组的 IL-18 蛋白,由 E. coli 表达,带有 N-His 标签。

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描述

IL-18 protein is a pro-inflammatory cytokine that is critical for epithelial barrier repair and immune regulation, especially in Th1 and NK cell responses. IL18R1 and IL18RAP combine to form a signaling ternary complex that activates NF-kappa-B and induces inflammatory mediators. IL-18 Protein, Dog (His) is the recombinant dog-derived IL-18 protein, expressed by E. coli , with N-His labeled tag.

研究背景

IL-18 蛋白是一种促炎细胞因子,在上皮屏障修复和调节免疫反应中发挥着至关重要的作用,特别是在极化辅助性 T 1 (Th1) 细胞和自然杀伤 (NK) 细胞免疫反应中。它与 IL18R1 和 IL18RAP 结合后形成信号三元复合物,激活 NF-kappa-B 并触发炎症介质的合成。IL-18 还与 IL-12/IL-12 协同作用,诱导 Th1 细胞和 NK 细胞合成 IFNG。此外,IL-18 参与细胞焦亡下游的炎症转导,其成熟形式通过gasdermin-D (GSDMD) 孔选择性释放到细胞外环境中。它与质膜上的配体结合受体亚基IL18R1和信号受体亚基IL18RAP形成三元复合物,其与货物受体TMED10的相互作用介导从细胞质易位至ERGIC(内质网-高尔基中间室)进行分泌。

种属

Dog

表达系统

E. coli

标签

N-His

蛋白编号

Q9XSR0 (Y37-S193)

基因 ID
蛋白结构
N-term
His
IL-18 (Y37-S193)
Accession # Q9XSR0
C-term
同用名
IL18; IGIF; Interleukin-18; IL-18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
氨基酸序列

YFGKLEPKLSIIRNLNDQVLFVNEGNQPVFEDMPDSDCTDNAPHTIFIIYMYKDSLTRGLAVTISVKYKTMSTLSCKNKTISFQKMSPPDSINDEGNDIIFFQRSVPGHDDKIQFESSLYKGHFLACKKENDLFKLILKDKDENGDKSIMFTVQNKS

分子量

Approximately 22.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IL-18 Protein, Dog (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-18 Protein, Dog (His)
目录号:
HY-P71657
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