IL-18R alpha 蛋白, Rat (HEK293, Fc)

IL-18R alpha (interleukin-18 receptor alpha) is an interleukin receptor of the immunoglobulin superfamily. IL-18 forms a signalling complex with the IL-18 receptor α (Rα) and β (Rβ) chains at the plasma membrane, which induces multiple inflammatory cytokines^[1]. IL-18R complex recruits the IL-1R- activating kinase and TNF- associated factor 6, which phosphorylates nuclear factor kβ- inducing kinase, with subsequent activation of nuclear factor kβ^[2]. IL-18R alpha inhibits the production of IFN-γ stimulated with the combination of rhIL-2 and rhIL-18^[3]. IL-18R alpha Protein, Rat (HEK293, Fc) is a recombinant protein with hFc tag and is produced by HEK293 cells.

IL-18R α 在 CD4⁺ 和 CD8⁺ T 细胞上表达，但在幼稚 T 细胞^[4]上不表达。
人IL-18Rα蛋白的氨基酸序列与小鼠IL-18Rα蛋白的同源性较低。
IL-18Rα是IL-18的受体。IL-18 分泌到细胞外，并逐步与免疫细胞质膜上的 IL-18 受体 α (Rα) 以及 IL-18 受体 β (Rβ) 结合。IL-18/IL-18Rα/IL-18Rβ 三元复合物形成并置 IL-18Rα 和 IL-18Rβ 的细胞内 Toll-Interleukin-1 受体结构域。然后，接头分子骨髓分化因子 88 (MyD88) 可能在 TRAM 的帮助下被招募。MyD88 进一步与 IL-1 受体相关激酶 (IRAK) 4 和 IRAK1/2 相互作用，形成称为 Myddosome 的大分子组装体，随后通过 TRAF6 激活 IKK。最后，信号激活 NF-κB 和丝裂原激活蛋白激酶通路7，从而上调各种炎症细胞因子的表达^[1]。
IL-18R α 与 IL-18 结合，IL-18 受体 β 形成信号复合物，诱导多种炎症细胞因子的表达^[1]。IL-18R α 抑制 rhIL-2 和 rhIL-18^[3] 联合刺激的 IFN-γ 产生。

Rat

HEK293

C-hFc

D3ZRM2 (A18-G326)

301365  [NCBI]

Approximately 105 kDa.

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Tsutsumi N, et al. The structural basis for receptor recognition of human interleukin-18. Nat Commun. 2014 Dec 15;5:5340.  [Content Brief]

  [2]. Watanabe M, et al. Predominant expression of 950delCAG of IL-18R alpha chain cDNA is associated with reduced IFN-gamma production and high serum IgE levels in atopic Japanese children. J Allergy Clin Immunol. 2002 Apr;109(4):669-75.  [Content Brief]

  [3]. Takei S, et al. Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Arthritis Res Ther. 2011 Mar 24;13(2):R52.  [Content Brief]

  [4]. Smeltz RB, et al. Regulation of interleukin (IL)-18 receptor alpha chain expression on CD4(+) T cells during T helper (Th)1/Th2 differentiation. Critical downregulatory role of IL-4. J Exp Med. 2001 Jul 16;194(2):143-53.  [Content Brief]