IL-1 beta 蛋白, Human (solution)

IL-1 beta protein is a potent proinflammatory cytokine that plays multiple roles in immune responses. It induces inflammatory events including prostaglandin synthesis, neutrophil activation, and cytokine production. IL-1 beta Protein, Human (solution) is the recombinant human-derived IL-1 beta protein, expressed by E. coli , with tag free. The total length of IL-1 beta Protein, Human (solution) is 153 a.a., with molecular weight of ~16.61 kDa.

IL-1 β 蛋白是一种有效的促炎细胞因子，因其在协调免疫反应中的多种作用而受到认可。IL-1β最初被认为是一种主要的内源性热原，可诱导一系列炎症事件，包括前列腺素合成、中性粒细胞流入和激活、T细胞和B细胞激活、细胞因子产生以及成纤维细胞增殖和胶原蛋白产生。它在免疫细胞分化中发挥着关键作用，促进 T 细胞的 Th17 分化，并与 IL-12 协同诱导辅助性 T 1 (Th1) 细胞合成 IFNG。此外，IL-1β 通过诱导 VEGF 产生，与 TNF 和 IL-6 协同作用，促进血管生成。值得注意的是，它在细胞焦亡下游炎症转导中发挥着关键作用，通过gasdermin-D (GSDMD) 孔特异性释放到细胞外环境中。在微生物感染的情况下，IL-1β充当皮肤中化脓性链球菌感染的传感器，被化脓性链球菌SpeB蛋白酶裂解和激活，导致炎症反应，从而在侵袭性皮肤感染期间抑制细菌生长。然而，SpeB 对 IL-1β 的裂解具有双重作用，通过破坏微生物群介导的定植抵抗来促进鼻咽链球菌感染。

Measured by its ability to induce NF-kB signaling in 293-IL1 Res cells. The ED₅₀ for this effect is 20-100 pg/mL.

Human

E. coli

Tag Free

P01584 (A117-S269)

3553  [NCBI]

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

Approximately 16.61 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.