IL-2R alpha/CD25 蛋白, Mouse (HEK293, His)

IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions^[1][2].IL-2R alpha/CD25 Protein, Mouse (HEK293, His) is a recombinant mouse IL-2R alpha protein with a His tag at the C-terminus and is expressed in HEK293 cells.

IL-2R α (CD25) 是一种 I 型膜蛋白。IL-2R α 在外周激活的 T 细胞和 B 细胞、三阴性胸腺细胞和骨髓前 B 细胞中表达。在高肿瘤调节性 T (Treg) 细胞中，IL-2R α 高表达，是 Treg 缺失的潜在靶点。在静息 T 细胞上检测不到 IL-2R α 的表达^[1][2][3]。
序列不同物种的IL-2Rα的氨基酸差异很大（人、大鼠和小鼠之间的相似性低于85%）。
IL-2Rα是高亲和力IL-2受体的重要组成部分，本身不具有信号转导活性。IL-2R α 通过增强 IL-2 与其受体复合物的结合发挥作用，并充当正反馈调节剂。IL-2是T淋巴细胞的主要生长因子，在T细胞免疫反应中发挥重要作用。IL-2R α 转录受三个正调节区 (PRR) 调节：PRRI、PRRII 和 PRRIII。PRRIII 是一种 IL-2 反应元件^[1][2]。
IL-2R α 调节 T 细胞生长，增强淋巴细胞活化和增殖。IL-2R α 参与预防 1 型糖尿病和癌症^[1][2][4]。

Mouse IL-2 (mIL-2)/CD25 (0.5 mg/kg; s.c.; twice a week for 1 week) induces a robust regulatory T cells (Tregs) expansion without showing signs of increase in the numbers of NK, CD4⁺ Foxp3, or CD8⁺ T cells or significant increase in proinflammatory cytokines^[5].
Mouse IL-2 (mIL-2)/CD25 (0.2-0.4 mg/kg; s.c.; twice a week for 1 week) demonstrates efficacy in inducing Treg expansion, CD25 upregulation, and inhibiting lupus nephritis based on the levels of proteinuria, autoantibody titers, and kidney histology scores in both NZB ◊ NZW and MRL/lpr mice^[5].

Measured in a cell proliferation assay using MO7e cells. The ED₅₀ for this effect is 0.8305 μg/mL in the presence of 30 ng/mL of recombinant human IL-2, corresponding to a specific activity is 1.2×10^3 units/mg.

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  Measured in a cell proliferation assay using MO7e cells. The ED₅₀ for this effect is 0.8305 μg/mL in the presence of 30 ng/mL of recombinant human IL-2, corresponding to a specific activity is 1.2×10³ units/mg.

Mouse

HEK293

C-6*His

P01590/NP_032393.3(E22-K236)

16184  [NCBI]

ELCLYDPPEVPNATFKALSYKNGTILNCECKRGFRRLKELVYMRCLGNSWSSNCQCTSNSHDKSRKQVTAQLEHQKEQQTTTDMQKPTQSMHQENLTGHCREPPPWKHEDSKRIYHFVEGQSVHYECIPGYKALQRGPAISICKMKCGKTGWTQPQLTCVDEREHHRFLASEESQGSRNSSPESETSCPITTTDFPQPTETTAMTETFVLTMEYK

40-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. H.Asao. Interleukin-2. Reference Module in Biomedical Sciences. 2014. ISBN 9780128012383.

  [2]. Kim HP, et al. The basis for IL-2-induced IL-2 receptor alpha chain gene regulation: importance of two widely separated IL-2 response elements. Immunity. 2001 Jul;15(1):159-72.  [Content Brief]

  [3]. Bell CJ, et al. Sustained in vivo signaling by long-lived IL-2 induces prolonged increases of regulatory T cells. J Autoimmun. 2015 Jan;56:66-80.  [Content Brief]

  [4]. Chistiakov DA, et al. The crucial role of IL-2/IL-2RA-mediated immune regulation in the pathogenesis of type 1 diabetes, an evidence coming from genetic and animal model studies. Immunol Lett. 2008 Jun 15;118(1):1-5.  [Content Brief]

  [5]. Xie JH, et al. Mouse IL-2/CD25 Fusion Protein Induces Regulatory T Cell Expansion and Immune Suppression in Preclinical Models of Systemic Lupus Erythematosus. J Immunol. 2021 Jul 1;207(1):34-43.  [Content Brief]