1. 重组蛋白
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. Multi-CSF/IL-3
  5. IL-3 蛋白, Cynomolgus (His)

IL-3 蛋白, Cynomolgus (His)

目录号: HY-P700859
产品使用指南

IL-3 蛋白由 T 淋巴细胞、肥大细胞和成骨细胞分泌,调节造血作用并激活嗜碱性粒细胞、嗜酸性粒细胞和单核细胞。它促进神经细胞增殖,抑制破骨细胞分化,并激活 JAK2-STAT5 信号传导。IL-3 还可激活 PI3K/AKT 和 ERK 通路,促进氧化应激期间细胞的存活。IL-3 蛋白, Cynomolgus (His) 是重组的 IL-3 蛋白,由 E. coli 表达,带有 N-His 标签。IL-3 蛋白, Cynomolgus (His) 全长 124 个氨基酸,分子量为 15.13 kDa。

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描述

IL-3 Protein, secreted by T-lymphocytes, mast cells, and osteoblastic cells, regulates hematopoiesis and activates basophils, eosinophils, and monocytes. It promotes neural cell proliferation, inhibits osteoclast differentiation, and activates JAK2-STAT5 signaling. IL-3 also activates PI3K/AKT and ERK pathways for cell survival during oxidative stress. IL-3 Protein, Cynomolgus (His) is the recombinant cynomolgus-derived IL-3 protein, expressed by E. coli , with N-His labeled tag. The total length of IL-3 Protein, Cynomolgus (His) is 124 a.a., with molecular weight of 15.13 kDa.

研究背景

IL-3蛋白是一种主要由活化的T淋巴细胞、肥大细胞和成骨细胞分泌的细胞因子,在调节造血祖细胞的产生和分化为特定细胞谱系中发挥着至关重要的作用。此外,IL-3 还可刺激成熟嗜碱性粒细胞、嗜酸性粒细胞和单核细胞的功能激活。它还在神经细胞增殖和存活中具有重要功能,并通过防止 NF-κ-B 核转位和激活来抑制破骨细胞分化,从而参与维持骨稳态。IL-3的生物学效应是通过由IL3RA亚基和IL3RB信号转导亚基组成的受体复合物介导的,从而导致JAK2激酶活性的快速激活以及随后STAT5介导的转录编程。此外,在非造血系统中,IL-3 通过激活涉及 PI3K/AKT 和 ERK 的途径,有助于氧化应激期间细胞的存活。

种属

Cynomolgus

表达系统

E. coli

标签

N-His

蛋白编号

G7P877 (A20-Q143)

基因 ID

/

蛋白结构
N-term
His
IL-3 (A20-Q143)
Accession # G7P877
C-term
同用名
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
分子量

15.13 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IL-3 Protein, Cynomolgus (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-3 Protein, Cynomolgus (His)
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