IL-3 蛋白, Human (HEK293, His)

IL-3 Protein, Human (HEK293, His) is a multipotent hematopoietic growth factor that can control blood formation. IL-3 Protein, Human (HEK293, His) is a recombinant human interleukin-3 (rhIL-3) expressed in HEK 293 cells with a His tag. rhIL-3 is also a weak inflammatory mediator. rhIL-3 can be used to improve states of hematopoietic failure^[1].

IL-3 在体外对红细胞、粒细胞巨噬细胞 (GM)、巨核细胞和肥大细胞谱系的细胞具有生长和分化诱导潜力。
此外，IL-3通过刺激单核细胞和嗜酸性粒细胞介导的吞噬作用和抗体依赖性细胞毒性（ADCC）、单核细胞M-CSF受体表达、肿瘤坏死因子（TNF）和M-CSF 合成、嗜碱性粒细胞产生细胞内组胺及其响应补体因子 C5a 的释放。
因此，IL-3 可能在需求增加的状态下充当激活炎症细胞的募集因子，而不是稳态造血过程中的主要调节元件^[1]。

The cell proliferation assay using TF-1 human erythroleukemic cells has an ED₅₀ value of 0.3-1.5 ng/mL.

Human

HEK293

C-6*His

P08700 (A20-F152)

3562  [NCBI]

APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF

17-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.

• [1]. A Lindemann, et al. Biologic effects of recombinant human interleukin-3 in vivo. J Clin Oncol. 1991 Dec;9(12):2120-7.  [Content Brief]