1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Biotinylated Proteins
  3. CSF & Receptors Stem Cell CD Proteins Dendritic Cell CD Proteins Endothelial cell CD Proteins
  4. IL-3R alpha/CD123
  5. IL-3R alpha/CD123 蛋白, Human (Biotinylated, HEK293, His-Avi)

IL-3R alpha/CD123 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P700747
产品使用指南

IL-3R α/CD123 是造血祖细胞、单核细胞和 B 淋巴细胞上表达的 IL3 的细胞表面受体,调节它们的产生和分化。IL-3R alpha/CD123 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 IL-3R α/CD123 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。IL-3R alpha/CD123 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 287 个氨基酸,分子量为 55-65 kDa。

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     可免费申领三个不同产品的试用装。

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描述

FITC-tagged IL-3R α/CD123 is a cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes, and B lymphocytes, regulating their production and differentiation. IL-3R alpha/CD123 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived IL-3R alpha/CD123 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of IL-3R alpha/CD123 Protein, Human (Biotinylated, HEK293, His-Avi) is 287 a.a., with molecular weight of 55-65 kDa.

研究背景

IL-3R α/CD123 蛋白作为 IL3 的细胞表面受体,在造血祖细胞、单核细胞和 B 淋巴细胞上表达,控制造血祖细胞向谱系限制细胞的产生和分化。在配体刺激后,它迅速与 IL3RB 发生异二聚化,导致效应蛋白(包括 JAK2 和 PI3K)磷酸化和激活。这些激活的途径在信号细胞增殖和分化中发挥着至关重要的作用。JAK2 激活进一步启动 STAT5 介导的转录程序,有助于细胞功能的调节。该受体与其配体 IL3 相互作用,形成由 α 和 β 亚基组成的异二聚体。值得注意的是,IL3、IL5 和 GM-CSF 受体共有 β 亚基。

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P26951-1 (T19-R305)

基因 ID
蛋白结构
N-term
IL-3Rα (T19-R305)
Accession # P26951-1
His-Avi
C-term
同用名
CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha
分子量

55-65 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-3R alpha/CD123 Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P700747
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