IL-4 蛋白, Bovine

IL-4 Protein, a key participant in B-cell activation and various cell types, acts as a costimulator of DNA synthesis. It induces class II MHC molecules on B-cells, enhances IgE and IgG1 secretion and expression, and regulates CD23 on lymphocytes and monocytes. IL-4 positively regulates IL31RA in macrophages and stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and inducing RUFY4. IL-4 Protein, Bovine is the recombinant bovine-derived IL-4 protein, expressed by E. coli , with tag free.

IL-4 蛋白作为关键调节剂积极参与多个 B 细胞激活过程并影响各种细胞类型。IL-4 作为共刺激剂，刺激 DNA 合成并诱导静息 B 细胞上 II 类 MHC 分子的表达。此外，它在增强 IgE 和 IgG1 的分泌和细胞表面表达方面发挥着关键作用，有助于免疫反应。IL-4 还调节淋巴细胞和单核细胞上 IgE 低亲和力 Fc 受体 (CD23) 的表达。除了对 B 细胞有影响外，IL-4 还能正向调节巨噬细胞中 IL31RA 的表达，进一步使其免疫调节功能多样化。此外，IL-4 通过干扰 mTORC1 信号传导和诱导 RUFY4 来刺激树突状细胞中的自噬，强调其复杂地参与不同免疫细胞类型的细胞过程。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.1303 ng/mL ,corresponding to a specific activity is 7.675×10⁶ U/mg

Bovine

E. coli

Tag Free

P30367 (H25-C135)

280824  [NCBI]

HKCDITLAEIIKTLNILTTRKNSCMELPVADVFAAPKNTTEKETFCRVGIELRRIYRSHTCLNKFLGGLDRNLNSLASKTCSVNEAKTSTSTLKDLLERLKTIMKEKYSKC

Approximately 12.7 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.