IL-4 蛋白, Cynomolgus (HEK293, His)

IL-4 protein has an important effect on B cell activation, acting as a costimulator for DNA synthesis and promoting the expression of class II MHC molecules on resting B cells. It enhances IgE and IgG1 secretion and cell surface expression while also regulating CD23 expression on lymphocytes and monocytes. IL-4 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-4 protein, expressed by HEK293 , with C-His labeled tag. The total length of IL-4 Protein, Cynomolgus (HEK293, His) is 129 a.a., with molecular weight of 23-28 kDa.

IL-4 蛋白在各种 B 细胞激活过程和其他细胞类型中发挥着关键作用，充当 DNA 合成的共刺激剂。此外，它还能促进静息 B 细胞上 II 类 MHC 分子的表达，并增强 IgE 和 IgG1 的分泌和细胞表面表达。IL-4 还调节淋巴细胞和单核细胞上 CD23（IgE 的低亲和力 Fc 受体）的表达。此外，它还可以通过干扰 mTORC1 信号传导和诱导 RUFY4 来正向调节巨噬细胞中 IL31RA 的表达并刺激树突状细胞中的自噬。

Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).

Cynomolgus

HEK293

C-His

P79339-1 (H25-S153)

107130068  [NCBI]

23-28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.