IL-4 蛋白, Human (HEK293)

IL-4 Protein, Human (HEK293) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of B-cells and T-cells. IL-4 Protein, Human (HEK293) is a recombinant human interleukin-4 (rhIL-4) expressed in HEK293 cells.

白介素 4 (IL-4) 是一种细胞因子，在调节炎症和免疫反应中发挥重要作用。它诱导幼稚辅助 T 细胞分化为 Th2 细胞。这种细胞因子与 IL-4 受体结合，而 IL-4 受体也与另一种细胞因子白细胞介素 13 (IL-13) 结合，这可能解释了 IL-4 和 IL-13 的重叠功能。IL-4最初被称为B细胞生长因子1(BSF1)，在活化的EL-4胸腺瘤细胞的上清液中被描述为可以共刺激用致屈服浓度的抗IgM^[1]。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ≤0.247 ng/mL, corresponding to a specific activity is ≥4.049×10⁷ units/mg.

• 
  The ED₅₀ is 0.05-0.247 ng/mL, as measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

Human

HEK293

Tag Free

P05112-1 (H25-S153)

3565  [NCBI]

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Approximately 16-21 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Le HV, et al. Isolation and characterization of multiple variants of recombinant human interleukin 4 expressed in mammalian cells. J Biol Chem. 1988 Aug 5;263(22):10817-23.  [Content Brief]

  [2]. Melih Ö Celik, et al. IL-4 induces M2 macrophages to produce sustained analgesia via opioids. JCI Insight. 2020 Feb 27;5(4):e133093.  [Content Brief]